Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
Monoclonal Mouse IgG1 Clone # 323519
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III Gly34-Glu251 Accession # P13726
Supplied in a saline solution containing BSA and Sodium Azide.
PerCP (Peridinin-chlorophyll Protein Complex)
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor PerCP‑conjugated Monoclonal Antibody (Catalog # FAB23391C) or (B) Mouse IgG1 PerCP Isotype Control (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a 46 kDa type I transmembrane protein that is part of the extrinsic pathway of coagulation. It functions as a protein cofactor/receptor for Coagulation Factor VII, which is synthesized in the liver and circulates in the plasma (1). Upon binding to TF, the inactive Factor VII is rapidly converted into activated Factor VIIa. The resulting 1:1 complex of Factor VIIa and TF activates the coagulation pathway, and, as a soluble form, plays an important coagulation-independent role in processes such as angiogenesis and M1 macrophage development (2-5). TF is synthesized as a 295 amino acid (aa) precursor that consists of a signal peptide (aa 1-32) plus a mature domain (aa 33-295). The mature region contain an extracellular region (aa 33-251), followed by a transmembrane segment (aa 252-274) and a cytoplasmic tail (aa 275-295) (6-9). TF is found on endothelial cells, monocytes, vascular smooth muscle cells, and bronchial epithelium (5, 10-12). Over aa 33-251, human and mouse TF share 58% aa sequence identity.
Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Gedding, J.E. and N. Mackman (2014) Thromb. Haemost. 111:570.
Hobbs, J.E. et al. (2007) Thromb. Res. 120 Suppl 2:S13.
Venkatasubramanian, S, et al. (2015) Eur. J. Immunol. Oct 16:e201545817.
Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
Morrissey, J.H. et al. (1987) Cell 50:129.
Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
Yang, X. et al. (2016) Thromb. Res. 137:211.
Arderiu, G. et al. (2015) Arterioscler. Thromb. Vasc. Biol. 35:348.
Mitchel, J.A. et al. (2015) Am. J. Resp. Cell Mol. Biol. Sept 25 [ePub ahead of print].
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