Detects human Coagulation Factor VII in Western blots. In this format, approximately 10% cross-reactivity with recombinant mouse Coagulation Factor VII is observed and less than 5% cross-reactivity with recombinant human Factor XA is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor VII (R&D Systems, Catalog # 2338-SE) Ala39-Pro444 Accession # NP_062562
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human Coagulation Factor VII (Catalog # 2338-SE)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Coagulation Factor VII in Human PBMCs. Coagulation Factor VII was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human Coagulation Factor VII Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2338) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor VII
Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively (1). Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, Factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). The cleavage and activation of Coagulation Factors VII, IX, and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not (1). The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor (3, 4). After a signal peptide (residues 1 to 38), the mature chain can be further processed into the light chain (residues 39-190) and the heavy chain (residues 191-444).
Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Hagen, F.S, et al. (1986) Proc. Natl. Acad. Sci. USA 83:2412.
O’Hara, P.J. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5158.
Coagulation Factor VII (Serum Prothrombin Conversion Accelerator)
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.