Detection of Human Cochlin by Western Blot.
Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Cochlin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5249) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cochlin at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cochlin (COCH/coagulation factor C homology; also Coch-5B2) is a 63-70 kDa, secreted member of the Cochlin-related glycoprotein family. It is expressed by type I and III fibrocytes of the auditory spiral limbus and constitutes the major noncollagenous component of inner ear ECM. Mature human Cochlin is 526 amino acids (aa) in length. It contains one LCCL (or FCH/factor C homologous) segment (aa 28-121) and two VWF-A domains (aa 165-346 and 367-537). Single aa changes at positions 66, 88 and 109 are detrimental to Cochlin deposition. Multiple splice variants exist. There are two potential alternate start sites at Met113 and Met194, an Ala-Lys substitution for aa 493 - 550, a 44 aa insertion after Thr244 and an 13 aa substitution for aa 343 - 550. Over aa 25-550, human Cochlin shares 96% aa identity with mouse Cochlin.
Entrez Gene IDs:
1690 (Human); 12810 (Mouse); 362735 (Rat)
coagulation factor C (Limulus polyphemus homolog); cochlin; coagulation factor C homolog, cochlin (Limulus polyphemus); COCH; COCH5B2; COCH-5B2COCH5B2; Cochlin; DFNA31; DFNA9
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