Human Cochlin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Cochlin in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Cochlin
    Glu25-Gln550
    Accession # O43405
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Cochlin by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Cochlin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5249) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cochlin at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cochlin

Cochlin (COCH/coagulation factor C homology; also Coch-5B2) is a 63-70 kDa, secreted member of the Cochlin-related glycoprotein family. It is expressed by type I and III fibrocytes of the auditory spiral limbus and constitutes the major noncollagenous component of inner ear ECM. Mature human Cochlin is 526 amino acids (aa) in length. It contains one LCCL (or FCH/factor C homologous) segment (aa 28-121) and two VWF-A domains (aa 165-346 and 367-537). Single aa changes at positions 66, 88 and 109 are detrimental to Cochlin deposition. Multiple splice variants exist. There are two potential alternate start sites at Met113 and Met194, an Ala-Lys substitution for aa 493 - 550, a 44 aa insertion after Thr244 and an 13 aa substitution for aa 343 - 550. Over aa 25-550, human Cochlin shares 96% aa identity with mouse Cochlin.

  • Entrez Gene IDs:
    1690 (Human); 12810 (Mouse); 362735 (Rat)
  • Alternate Names:
    coagulation factor C (Limulus polyphemus homolog); cochlin; coagulation factor C homolog, cochlin (Limulus polyphemus); COCH; COCH5B2; COCH-5B2COCH5B2; Cochlin; DFNA31; DFNA9
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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