Human Cochlin Antibody

Name: Human Cochlin Antibody
Brand: R&D Systems
SKU: AF5249

Catalog #: AF5249 Datasheet / COA / SDS

Discontinued Product

AF5249 has been discontinued.

View all Cochlin products.

Detection of Human Cochlin by Western Blot.

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All Cochlin Antibodies

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Human Cochlin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cochlin in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cochlin
Glu25-Gln550
Accession # O43405

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Cochlin by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Cochlin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5249) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cochlin at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cochlin

Cochlin (COCH/coagulation factor C homology; also Coch-5B2) is a 63-70 kDa, secreted member of the Cochlin-related glycoprotein family. It is expressed by type I and III fibrocytes of the auditory spiral limbus and constitutes the major noncollagenous component of inner ear ECM. Mature human Cochlin is 526 amino acids (aa) in length. It contains one LCCL (or FCH/factor C homologous) segment (aa 28-121) and two VWF-A domains (aa 165-346 and 367-537). Single aa changes at positions 66, 88 and 109 are detrimental to Cochlin deposition. Multiple splice variants exist. There are two potential alternate start sites at Met113 and Met194, an Ala-Lys substitution for aa 493 - 550, a 44 aa insertion after Thr244 and an 13 aa substitution for aa 343 - 550. Over aa 25-550, human Cochlin shares 96% aa identity with mouse Cochlin.

Entrez Gene IDs

1690 (Human); 12810 (Mouse); 362735 (Rat)

Alternate Names

coagulation factor C (Limulus polyphemus homolog); cochlin; coagulation factor C homolog, cochlin (Limulus polyphemus); COCH; COCH5B2; COCH-5B2COCH5B2; Cochlin; DFNA31; DFNA9