Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Complement Component C2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C2
Ala21-Leu752
Accession # P06681

Specificity

Detects human Complement Component C2 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) C1r, rhC1s, rhC3a, and rhC5a is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Complement Component C2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Complement Component C2 (Catalog # 1936-SE)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C2

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s (1). After activation by C1, the single-chain form of C2 (amino acid residues 21‑752) becomes two chains, which are referred to as C2A and C2B. C2A (residues 244‑752) consists of a vWF domain (residues 254‑452) and a serine protease domain (residues 466‑752). C2B (residues 21‑243) contains 3 Sushi (SCR) domains. C2A, then combines with complement factor 4B to generate the C3 or C5 convertase. The full‑length of human C2 was expressed, and the purified protein corresponded to the single-chain form with the peptidase activity.

References

  1. Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.

Alternate Names

C2

Entrez Gene IDs

717 (Human); 12263 (Mouse); 24231 (Rat)

Gene Symbol

C2

UniProt

P06681

Additional Complement Component C2 Products

Product Documents for Human Complement Component C2 Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Human Complement Component C2 Biotinylated Antibody

For research use only

Citations for Human Complement Component C2 Biotinylated Antibody

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