Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Complement Component C2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C2
Ala21-Leu752
Accession # P06681

Specificity

Detects human Complement Component C2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant mouse Complement Component C2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Complement Component C2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Component C2 (Catalog # 1936-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Complement Component C2 (Catalog # 1936-SE)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C2

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s (1). After activation by C1, the single-chain form of C2 (amino acid residues 21‑752) becomes two chains, which are referred to as C2A and C2B. C2A (residues 244‑752) consists of a vWF domain (residues 254‑452) and a serine protease domain (residues 466‑752). C2B (residues 21‑243) contains 3 Sushi (SCR) domains. C2A, then combines with complement factor 4B to generate the C3 or C5 convertase. The full‑length of human C2 was expressed, and the purified protein corresponded to the single-chain form with the peptidase activity.

References

  1. Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.

Alternate Names

C2

Entrez Gene IDs

717 (Human); 12263 (Mouse); 24231 (Rat)

Gene Symbol

C2

UniProt

P06681

Additional Complement Component C2 Products

Product Documents for Human Complement Component C2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Component C2 Antibody

For research use only

Citations for Human Complement Component C2 Antibody

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