Human Complement Factor D/Adipsin Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (3)
Supplemental Products
Reviews (3)

Human Complement Factor D/Adipsin Antibody Summary

Species Reactivity
Detects human Complement Factor D in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Complement Factor D is observed, and less than 1% cross-reactivity with recombinant human (rh) Complement Factor B and rhComplement Factor I is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Complement Factor D
Accession # P00746
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Complement Factor D/Adipsin (Catalog # 1824-SE)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Complement Factor D/Adipsin (Catalog # 1824-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor D/Adipsin

Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).

  1. White, R.T. et al. (1992) J. Biol. Chem. 267:9210.
  2. Taylor, F.R. et al. (1999) Biochemistry 38:2849.
  3. Kim, S. et al. (1995) J. Biol. Chem. 270:24399.
Entrez Gene IDs
1675 (Human); 11537 (Mouse); 54249 (Rat)
Alternate Names
Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC; PFD; Properdin factor DADIPSIN

Product Datasheets

You must select a language.


Citations for Human Complement Factor D/Adipsin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Induction of Ocular Complement Activation by Inflammatory Stimuli and Intraocular Inhibition of Complement Factor D in Animal Models
    Authors: MA Crowley, O Delgado, A Will-Orreg, NM Buchanan, K Anderson, BD Jaffee, TP Dryja, SM Liao
    Invest. Ophthalmol. Vis. Sci., 2018;59(2):940-951.
  2. Analysis of Factor D Isoforms in Malpuech-Michels-Mingarelli-Carnevale Patients Highlights the Role of MASP-3 as a Maturase in the Alternative Pathway of Complement
    Authors: R Pihl, L Jensen, AG Hansen, IB Thøgersen, S Andres, F Dagnæs-Han, K Oexle, JJ Enghild, S Thiel
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Mannan-binding lectin-associated serine protease (MASP)-1 is crucial for lectin pathway activation in human serum, whereas neither MASP-1 nor MASP-3 is required for alternative pathway function.
    Authors: Degn S, Jensen L, Hansen A, Duman D, Tekin M, Jensenius J, Thiel S
    J Immunol, 2012;189(8):3957-69.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Complement Factor D/Adipsin Antibody

Average Rating: 5 (Based on 3 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human Complement Factor D/Adipsin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human Complement Factor D/Adipsin Antibody
By Anonymous on 05/12/2021
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Used this antibody as the detector in a 2-step adipsin immunoassay.

Human Complement Factor D/Adipsin Antibody
By Anonymous on 08/27/2017
Application: ELISA Sample Tested: 293T human embryonic kidney cell line,CHO Chinese hamster ovary cell line Species: Human

Human Complement Factor D/Adipsin Antibody
By Sayaka Sato on 05/16/2016
Application: WB Sample Tested: Purified protein Species: Human