Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rabbit

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Complement Factor D/Adipsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor D
Ile26-Ala253
Accession # P00746

Specificity

Detects human Complement Factor D in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Complement Factor D is observed, and less than 1% cross-reactivity with recombinant human (rh) Complement Factor B and rhComplement Factor I is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Complement Factor D/Adipsin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Factor D/Adipsin (Catalog # 1824-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Complement Factor D/Adipsin (Catalog # 1824-SE)

Reviewed Applications

Read 4 reviews rated 5 using AF1824 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor D/Adipsin

Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).

References

  1. White, R.T. et al. (1992) J. Biol. Chem. 267:9210.
  2. Taylor, F.R. et al. (1999) Biochemistry 38:2849.
  3. Kim, S. et al. (1995) J. Biol. Chem. 270:24399.

Alternate Names

Adipsin, ADN, AMBP-1, CFD, PFD

Entrez Gene IDs

1675 (Human); 11537 (Mouse); 54249 (Rat)

Gene Symbol

CFD

UniProt

P00746

Additional Complement Factor D/Adipsin Products

Product Documents for Human Complement Factor D/Adipsin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Factor D/Adipsin Antibody

For research use only

Related Research Areas

Complement Pathway

Citations for Human Complement Factor D/Adipsin Antibody

Customer Reviews for Human Complement Factor D/Adipsin Antibody (4)

5 out of 5
4 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
0%

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Showing  1 - 4 of 4 reviews Showing All
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  • Human Complement Factor D/Adipsin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Serum and Recombinant protein
    Species: Human
    Verified Customer | Posted 03/22/2024
    Human Complement Factor D/Adipsin Antibody AF1824
  • Human Complement Factor D/Adipsin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 05/12/2021
    Used this antibody as the detector in a 2-step adipsin immunoassay.
    Human Complement Factor D/Adipsin Antibody AF1824
  • Human Complement Factor D/Adipsin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: 293T human embryonic kidney cell line and CHO Chinese hamster ovary cell line
    Species: Human
    Verified Customer | Posted 08/27/2017
  • Human Complement Factor D/Adipsin Antibody
    Name: Sayaka Sato
    Application: Western Blot
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 05/16/2016
    Human Complement Factor D/Adipsin Antibody AF1824

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