Human Corneodesmosin Antibody

Catalog # Availability Size / Price Qty
AF5725
AF5725-SP
Corneodesmosin in Human Skin.
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Product Details
Citations (3)
FAQs
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Human Corneodesmosin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Corneodesmosin in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Corneodesmosin
Lys33-Pro529 (Ser153 del)
Accession # NP_001255
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Corneodesmosin in Human Skin. View Larger

Corneodesmosin in Human Skin. Corneodesmosin was detected in immersion fixed paraffin-embedded sections of human skin using Sheep Anti-Human Corneodesmosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5725) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human Corneodesmosin by Western Blot. View Larger

Detection of Human Corneodesmosin by Western Blot. Western blot shows lysates of NCI‑N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Corneodesmosin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5725) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Corneodesmosin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Corneodesmosin

Corneodesmosin, also known as CDSN and the S gene product, is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin (1). It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes (2-4). It is also expressed in the inner root sheath of hair follicles (5, 6). Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains (2, 7). Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes (8, 9). Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness (6, 10). Corneodesmosin is secreted by keratinocytes as a 52-56 kDa molecule which is then subjected to repeated sequential N- and C-terminal proteolysis (11). Species of 46, 43, 36, and 15 kDa are present in corneocytes (7, 11). Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin’s ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin (8, 9). Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention (12). Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp (13).

References
  1. Pierard, G.E. et al. (2000) Int. J. Mol. Med. 6:217.
  2. Zhou, Y. and D.D. Chaplin (1993) Proc. Natl. Acad. Sci. USA 90:9470.
  3. Haftek, M. et al. (1997) Br. J. Dermatol. 137:864.
  4. Simon, M. et al. (1997) J. Biol. Chem. 272:31770.
  5. Mils, V. et al. (1992) J. Histochem. Cytochem. 40:1329.
  6. Matsumoto, M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6720.
  7. Guerrin, M. et al. (1998) J. Biol. Chem. 273:22640.
  8. Jonca, N. et al. (2002) J. Biol. Chem. 277:5024.
  9. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:747.
  10. Leclerc, E.A. et al. (2009) J. Cell Sci. 122:2699.
  11. Simon, M. et al. (2001) J. Biol. Chem. 276:20292.
  12. Simon, M. et al. (2008) Br. J. Dermatol. 159:77.
  13. Levy-Nissenbaum, E. et al. (2003) Nat. Genet. 34:151.
Entrez Gene IDs
1041 (Human); 386463 (Mouse)
Alternate Names
CDSN; Corneodesmosin; D6S586E; differentiated keratinocyte S protein; HTSS; S protein; S

Product Datasheets

Citations for Human Corneodesmosin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The caspase-1 inhibitor CARD18 is specifically expressed during late differentiation of keratinocytes and its expression is lost in lichen planus
    Authors: H Qin, J Jin, H Fischer, M Mildner, M Gschwandtn, V Mlitz, L Eckhart, E Tschachler
    J. Dermatol. Sci., 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation
    Authors: E Jacquin, S Leclerc-Me, C Judon, E Blanchard, S Fraitag, O Florey
    Autophagy, 2017;0(0):1-14.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Callus formation is associated with hyperproliferation and incomplete differentiation of keratinocytes, and increased expression of adhesion molecules.
    Authors: Kim SH, Kim S
    Br. J. Dermatol., 2010;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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