Human COUP‑TF II/NR2F2 Antibody

R&D Systems | Catalog # PP-H7147-00

R&D Systems
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Primate, Primate - Chlorocebus aethiops (African Green Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Bioassay, In Situ Hybridization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H7147
View all COUP-TF II/NR2F2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human COUP-TF II/NR2F2
Amino Acids 43-64

Specificity

This antibody specifically recognizes human COUP-TF II and cross-reacts with rat COUP-TF II. This antibody does not cross-react with human COUP-TF I or EAR2. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human COUP‑TF II/NR2F2 Antibody

Detection of Human COUP-TF II/NR2F2 by Knockdown Validated

Detection of Human COUP-TF II/NR2F2 by Knockdown Validated

Reduction of COUP-TFII or nucleolin decreases RAR beta 2 transcription in MCF-7 cells.MCF-7 (A) and T47D (B) cells were transfected with control siRNA or an siRNA targeting nucleolin for 48 h. T47D cells were treated with EtOH or 1 µM atRA for 24 h. Q-PCR for nucleolin (NCL) and RARB2. Values are the average of triplicates. C, Western blot showing COUP-TFII and RAR beta 2 expression after transfection with siCOUP-TFII. Values are relative to beta -actin. MCF-7 were transfected with siControl or siCOUP-TFII for 48 h and treated with 1 µM atRA for 6 h. Q-PCR was also performed for RRIG1. P<0.001 * versus control or ** versus atRA. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0038278), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human COUP-TF II/NR2F2 by Knockdown Validated

Detection of Human COUP-TF II/NR2F2 by Knockdown Validated

Loss of miR-101 promotes cancer metastasis through de-repression of COUP-TFII expression.(a) LNCaP and PC3 cells were individually treated with 50 nM of miR-101 and miR-27a mimic for 48 h and then invasion assays were performed for an additional 16 h (n=3). *P<0.05 (two-sided Student's t-test) compared with the control group. (b) Representative western blot showed the levels of COUP-TFII, E-cadherin, vimentin and beta -actin in PC3 cells stably knocked down by COUP-TFII. (c) LNCaP and PC3 cells stably knocked-down of COUP-TFII were used to perform cell invasion assay. Invaded cells were stained and counted (lower panel) (n=3). Representative western blot showed the knockdown efficiency of COUP-TFII (upper panel). *: P<0.05 (two-sided Student's t-test) compared with control group. (d) PC3 cells carrying an inducible miR-101 in the absence and presence of doxycycline, and re-expression of COUP-TFII were used to perform invasion assays (n=3). Representative western blot showed the levels of COUP-TFII and beta -actin (left). Invaded cells were counted and results are shown in the right panel. *: P<0.05 (two-sided Student's t-test) (e) 22RV-1 cells were treated with miR-101 inhibitor (antisense RNA) in conjunction with knockdown of COUP-TFII and used for invasion assays (n=3). Representative western blot show the levels of COUP-TFII and beta -actin (left). Invaded cells were counted and result is shown in the right panel. *: P<0.05 (two-sided Student's t-test). (f) LNCaP cells containing a construct with inducible expression of COUP-TFII shRNA and constructs expressing with anti-miR-101 and anti-mir-27a were orthotopically injected into NOD-SCID mouse prostate. In addition these cells also contain a luciferase reporter to detect cancer cells. After the tumour size was bigger than 50 mm3, drinking water with or without doxycycline (1 mg ml−1) was given to the mice for 6 weeks to induce the COUP-TFII shRNA to repress COUP-TFII expression. Representative bioluminescence results show the status of tumour growth in different groups. (g) Quantification result of luminance from IVIS (control: n=5; anti-miR-101/27a(−): n=6; anti-miR-101/27a(+): n=7. *: P<0.05 (two-sided Student's t-test). (h) Immunohistochemical stain showed the metastatic tumour cells located in the mouse lymph node are positive for AR. Scale bar: 100 μM (low power); and 200 μM (high power). SCID, severe combined immunodeficient. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms11418), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human COUP-TF II/NR2F2 by Knockdown Validated

Detection of Human COUP-TF II/NR2F2 by Knockdown Validated

Loss of miR-101 promotes cancer metastasis through de-repression of COUP-TFII expression.(a) LNCaP and PC3 cells were individually treated with 50 nM of miR-101 and miR-27a mimic for 48 h and then invasion assays were performed for an additional 16 h (n=3). *P<0.05 (two-sided Student's t-test) compared with the control group. (b) Representative western blot showed the levels of COUP-TFII, E-cadherin, vimentin and beta -actin in PC3 cells stably knocked down by COUP-TFII. (c) LNCaP and PC3 cells stably knocked-down of COUP-TFII were used to perform cell invasion assay. Invaded cells were stained and counted (lower panel) (n=3). Representative western blot showed the knockdown efficiency of COUP-TFII (upper panel). *: P<0.05 (two-sided Student's t-test) compared with control group. (d) PC3 cells carrying an inducible miR-101 in the absence and presence of doxycycline, and re-expression of COUP-TFII were used to perform invasion assays (n=3). Representative western blot showed the levels of COUP-TFII and beta -actin (left). Invaded cells were counted and results are shown in the right panel. *: P<0.05 (two-sided Student's t-test) (e) 22RV-1 cells were treated with miR-101 inhibitor (antisense RNA) in conjunction with knockdown of COUP-TFII and used for invasion assays (n=3). Representative western blot show the levels of COUP-TFII and beta -actin (left). Invaded cells were counted and result is shown in the right panel. *: P<0.05 (two-sided Student's t-test). (f) LNCaP cells containing a construct with inducible expression of COUP-TFII shRNA and constructs expressing with anti-miR-101 and anti-mir-27a were orthotopically injected into NOD-SCID mouse prostate. In addition these cells also contain a luciferase reporter to detect cancer cells. After the tumour size was bigger than 50 mm3, drinking water with or without doxycycline (1 mg ml−1) was given to the mice for 6 weeks to induce the COUP-TFII shRNA to repress COUP-TFII expression. Representative bioluminescence results show the status of tumour growth in different groups. (g) Quantification result of luminance from IVIS (control: n=5; anti-miR-101/27a(−): n=6; anti-miR-101/27a(+): n=7. *: P<0.05 (two-sided Student's t-test). (h) Immunohistochemical stain showed the metastatic tumour cells located in the mouse lymph node are positive for AR. Scale bar: 100 μM (low power); and 200 μM (high power). SCID, severe combined immunodeficient. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms11418), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human COUP-TF II/NR2F2 by Knockdown Validated

Detection of Human COUP-TF II/NR2F2 by Knockdown Validated

FOXM1 and CENPF are downstream targets of COUP-TFII in PCa.(a) Gene expression profile of COUP-TFII knockdown PC3 cells (GSE33182) was downloaded from GEO database and analysed using GenePattern. Genes with P<0.05, FOLD CHANGE >1.5-fold are represented as a heatmap. The gene symbol of FOXM1 and CENPF genes after knockdown of COUP-TFII are annotated in red colour. The others were FOXM1 and CENPF co-regulated genes annotated in black colour. siCtrl: knockdown scramble control; siCII: knockdown of COUP-TFII. (b) A representative western blot shows the level of COUP-TFII in PC3 cells transfected with two different siRNAs against COUP-TFII for 72 h (upper panel). Levels of FOXM1 and CENPF are quantified by RT-qPCR (lower panel) (n=3). *P<0.05 (two-sided Student's t-test) compared with the control group (siCON). (c) A cartoon shows the FOXM1 and CENPF loci. COUP-TFII-binding sites are annotated by a black rectangle (upper panel). ChIP results are shown in the PC3 cells treated with control and two different siRNAs against COUP-TFII for 72 h (lower panel) (n=3). *P<0.05 (two-sided Student's t-test) compared with control. (d) FOXM1 and CENPF promoter activities were measured in PC3 cells carrying an inducible COUP-TFII gene in the absence or the presence of doxycycline for 48 h (n=3). *P<0.05 (two-sided Student's t-test). (e) FOXM1 and CENPF promoter activities were measured in control or stably knocked-down COUP-TFII in PC3 cells for 48 h (n=3). *P<0.05 (two-sided Student's t-test). (f) PC3 cells carrying with inducible COUP-TFII-Flag gene were treated with or without doxycycline for 48 h and then transfected with individual siRNA against FOXM1 and CENPF or double knockdown of FOXM1 and CENPF for an additional 48 h. western blot (left panel) shows the level of COUP-TFII, CENPF and FOXM1 expression. Invasion assay (right panel) was performed by using those cells (n=3). *P<0.05 (two-sided Student's t-test) compared with control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms11418), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human COUP-TF II/NR2F2 by Western Blot

Detection of Human COUP-TF II/NR2F2 by Western Blot

Loss of miR-101 promotes cancer metastasis through de-repression of COUP-TFII expression. (d) PC3 cells carrying an inducible miR-101 in the absence&presence of doxycycline,&re-expression of COUP-TFII used to perform invasion assays (n=3). Representative WB showed the levels of COUP-TFII& beta -actin (left). Invaded cells counted&results are shown in the right panel. *: P<0.05 (two-sided Student's t-test). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27108958), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human COUP-TF II/NR2F2 by Western Blot

Detection of Human COUP-TF II/NR2F2 by Western Blot

FOXM1&CENPF are downstream targets of COUP-TFII in PCa. (f) PC3 cells carrying with inducible COUP-TFII-Flag gene treated with or without doxycycline for 48 h&then transfected with individual siRNA against FOXM1&CENPF or double knockdown of FOXM1&CENPF for an additional 48 h. WB (left panel) shows the level of COUP-TFII, CENPF&FOXM1 expression. Invasion assay (right panel) was performed by using those cells (n=3). *P<0.05 (two-sided Student's t-test) compared with control. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27108958), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human COUP-TF II/NR2F2 by Western Blot

Detection of Human COUP-TF II/NR2F2 by Western Blot

Loss of miR-101 promotes cancer metastasis through de-repression of COUP-TFII expression.(e) 22RV-1 cells treated with miR-101 inhibitor (antisense RNA) in conjunction with knockdown of COUP-TFII&used for invasion assays (n=3). Representative WB show the levels of COUP-TFII& beta -actin (left). Invaded cells counted&result is shown in the right panel. *: P<0.05 (two-sided Student's t-test). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27108958), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human COUP‑TF II/NR2F2 Antibody

Application
Recommended Usage

Immunohistochemistry

This antibody can be used at 10 ug/mL with the appropriate secondary reagents to detect human COUP-TF II alpha.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human COUP-TF II.

Reviewed Applications

Read 6 reviews rated 4.5 using PP-H7147-00 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70° C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: COUP-TF II/NR2F2

Chicken ovalbumin upstream promoter transcription factor II (COUP-TF II, ARP-1, COUP-TFB; NR2F2) is a member of the orphan nuclear receptorfamily. COUP-TF II is expressed in the tongue, follicles of vibrissae, cochIea and in stroma of the nasal septum. COUP-TF II has roles in angiogenesis, vascular remodeling and heart development. COUP-TFs were shown to interact with a number of other nuclear receptors.

Long Name

COUP Transcription Factor 1/Nuclear Receptor Subfamily 2, Group F, Member 2

Alternate Names

COUPTF II, NR2F2, SVP40

Entrez Gene IDs

7026 (Human)

Gene Symbol

NR2F2

Additional COUP-TF II/NR2F2 Products

Product Documents for Human COUP‑TF II/NR2F2 Antibody

Certificate of Analysis

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Product Specific Notices for Human COUP‑TF II/NR2F2 Antibody

For research use only

Citations for Human COUP‑TF II/NR2F2 Antibody

Customer Reviews for Human COUP‑TF II/NR2F2 Antibody (6)

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Customer Images

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  • Human COUP-TF II/NR2F2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovary (developing oocytes)
    Species: Mouse
    Verified Customer | Posted 11/15/2024
    Worked on mouse organoid, concentration 1:200, IHC-P
    Human COUP‑TF II/NR2F2 Antibody PP-H7147-00
  • Human COUP-TF II/NR2F2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Ovary tissue
    Species: Mouse
    Verified Customer | Posted 11/08/2024
    The antibody was tested on a paraffin-embedded section of a mouse P14 ovary, with successful staining observed at a dilution of 1:200. Yellow (Nr2F2) Pink (Stella)
    Human COUP‑TF II/NR2F2 Antibody PP-H7147-00
  • Human COUP-TF II/NR2F2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney angiomyolipoma
    Species: Human
    Verified Customer | Posted 08/17/2021
    Human COUP‑TF II/NR2F2 Antibody PP-H7147-00
  • Human COUP-TF II/NR2F2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: E17 coronal brain section
    Species: Mouse
    Verified Customer | Posted 05/13/2019
    E17 frozen mouse embryonic brain section was stained with Coup-TFII antibody (green) and dapi was used for contrast.
    Brain sections were antigen retrieved with 10mM sodium citrate (pH6) buffer and then blocked with 10% donkey serum in PBST. primary and secondary antibodies were prepared in the same blocking buffer.
    Human COUP‑TF II/NR2F2 Antibody PP-H7147-00
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 22253897
    Species: Rat
    Verified Customer | Posted 02/25/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22615892
    Species: Rat
    Verified Customer | Posted 02/25/2015

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