Human CRIM1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1917

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Human CRIM1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CRIM1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRIM1
Leu35-Ser939
Accession # Q9NZV1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CRIM1 (Catalog # 1917-CM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRIM1

Cysteine rich motor neuron 1 (CRIM1) is a type I transmembrane glycoprotein of the chordin-like cysteine-rich repeat (CRR) family of BMP inhibitors (1‑4). The ~130 kDa, 1036 amino acid (aa) CRIM1 contains a 34 aa signal sequence, a 905 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 76 aa cytoplasmic domain. The ECD includes an N-terminal IGF-binding protein-like motif and six chordin-like von Willebrand C-type CRRs. The ECD can be released from the cell, presumably by proteolytic processing (4). Human CRIM1 ECD shows 88%, 88%, 91%, 86%, 87%, 83% and 72% aa identity with mouse, rat, dog, cow, opossum, chick and zebrafish CRIM1 ECD, respectively. CRIM1 can interact with TGF-beta family ligands, including BMPs 2, 4 and 7, via its CRR domains (4). It binds BMPs intracellularly and antagonizes them by lowering their expression, processing and secretion (4). CRIM1 is expressed in the developing spinal cord in the floor plate and developing motor neurons (1). It is also expressed by perivascular smooth muscle cells and aligns at points of cell-cell contact during endothelial cell capillary formation (2). Endothelial cell expression in vitro appears to be specific to cells that are adherent and growing (2). CRIM1 is also expressed in a spatially and temporally restricted manner in the developing lens, limbs, kidney, teeth and testis (5). Studies where CRIM1 expression is manipulated in developing mouse, chick and zebrafish support its involvement in regulation of vascular and somitic development and organogenesis (5‑7).

References
  1. Kolle, G. et al. (2000), Mech. Dev. 90:181.
  2. Glienke, J. et al. (2002) Mech. Dev. 119:165.
  3. Abreu, J. G. et al. (2002) Gene 287:39.
  4. Wilkinson, L. et al. (2003) J. Biol. Chem. 278:34181.
  5. Pennisi, D. J. et al. (2007) Dev. Dyn. 236:502.
  6. Kolle, G. et al. (2003) Dev. Dyn. 226:107.
  7. Kinna, G. et al. (2006) Mech. Dev. 123:277.
Long Name
Cysteine Rich Motor Neuron 1 Protein
Entrez Gene IDs
51232 (Human); 50766 (Mouse)
Alternate Names
CRIM1; CRIM-1; cysteine rich transmembrane BMP regulator 1 (chordin-like); cysteine-rich motor neuron 1 protein; cysteine-rich motor neuron 1; Cysteine-rich repeat-containing protein S52; MGC138194; S52

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