Detection of CX3CL1/Fractalkine in NS0 Cell Line Transfected with His-tagged Human CX3CL1 by Flow Cytometry.
NS0 cell line transfected with His-tagged human CX3CL1 was stained with Mouse Anti-His Tag APC‑conjugated Monoclonal Antibody (Catalog # IC050A) and either (A) Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain PE‑conjugated Monoclonal Antibody (Catalog # IC365P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C
cysteine motif near the amino-terminus and is the first member of a
fourth branch of the chemokine superfamily. Unlike other known
chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine
domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a
397 amino acid (aa) residue membrane protein with a 24 aa residue
predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa
residue stalk region containing 17 degenerate mucin-like repeats, a
19 aa residue transmembrane segment and a 37 aa residue cytoplasmic
domain. The extracellular domain of human CX3CL1 can be released,
possibly by proteolysis at the dibasic cleavage site proximal to the
membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in
various tissues including the brain and heart. The expression of CX3CL1
was also reported to be up-regulated in endothelial cells and microglia
by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote
adhesion of leukocytes. The soluble chemokine domain of human CX3CL1
was reported to be chemotactic for T cells and monocytes while the
soluble chemokine domain of mouse CX3CL1 was reported to chemoattract
neutrophils and T-lymphocytes but not monocytes.
Pan, Y. et al. (1997) Nature 387:611.
Bazan, J.F. et al. (1997) Nature 385:640.
Mackay, C.R. (1997) Current Biology 7:R384.
Entrez Gene IDs:
6376 (Human); 20312 (Mouse); 89808 (Rat)
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1
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but also provides information about sample types, species, and experimental conditions.
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