Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 51637
View all CX3CL1/Fractalkine Primary Antibodies »

Product Specifications

Immunogen

NS0-derived and E.coli-derived Recombinant Human Fractalkine
Gln25-Arg339
Accession # P78423

Specificity

Detects human CX3CL1 in ELISAs and Western blots.  In ELISAs, this antibody shows approximately 8% cross-reactivity with recombinant mouse (rm) CXCL6 and less than 0.2% cross-reactivity with rhCXCL6, rhCXCL9, and rrCX3CL1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # 365-FR)

Human CX3CL1/Fractalkine Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # MAB3652)
  • Standard: Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog # 365-FR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CX3CL1/Fractalkine

Fractalkine is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Alternate Names

FKN, Fractalkine, Neurotactin

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Gene Symbol

CX3CL1

UniProt

P78423

Additional CX3CL1/Fractalkine Products

Product Documents for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

For research use only

Citations for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

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