Human CXCL9/MIG PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC392P
Detection of CXCL9/MIG in rhIFN gamma -treated THP‑1 Human Cell Line by Flow Cytometry.
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Human CXCL9/MIG PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL9/MIG in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant mouse (rm) CXCL9 or recombinant human CXCL10.
Source
Monoclonal Mouse IgG1 Clone # 49106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CXCL9/MIG
Thr23-Thr125
Accession # Q07325
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of CXCL9/MIG antibody in rhIFN?-treated THP-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CXCL9/MIG in rhIFN gamma -treated THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was treated for 24 hr with 100 U/mL rhIFN gamma then stained with Mouse Anti-Human CXCL9/MIG PE-conjugated Monoclonal Antibody (Catalog # IC392P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CXCL9/MIG

CXCL9, a member of the alpha  subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide that is cleaved to yield a 103 aa residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic aa residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. CXCL9 is a homodimer and will heterodimerize with CXCL12. It is reported to bind to CXCR3, CXCR6 and CCR3. Over amino acids (aa) 23-125, human and mouse CXCl9 share 67% aa sequence identity. 

Entrez Gene IDs
4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma

Product Datasheets

Citation for Human CXCL9/MIG PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
    Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
    J Allergy Clin Immunol, 2014;133(1):172-80.e1-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Chip Cytometry

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