Human CXCL9/MIG Antibody

Catalog # Availability Size / Price Qty
MAB392-SP
MAB392-100
MAB392-500
CXCL9/MIG in THP‑1 Human Cell Line.
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (2)

Human CXCL9/MIG Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL9/MIG in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant mouse (rm) CXCL9, recombinant human CXCL10.
Source
Monoclonal Mouse IgG1 Clone # 49106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CXCL9/MIG
Thr23-Thr125
Accession # Q07325
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CXCL9/MIG (Catalog # 392-MG)
under non-reducing conditions only
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
THP-1 cells treated with Recombinant Human IFN‑ gamma (Catalog # 285‑IF), fixed with paraformaldehyde, and permeabilized with saponin

Human CXCL9/MIG Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CXCL9/MIG Biotinylated Antibody (Catalog # BAF392)

Standard: Recombinant Human CXCL9/MIG Protein (Catalog # 392-MG)

Neutralization
Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 0.5-4 µg/mL in the presence of 0.25 µg/mL Recombinant Human CXCL9/MIG.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry View Larger

CXCL9/MIG in THP‑1 Human Cell Line. CXCL9/MIG was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line treated with IFN gamma (positive staining) and THP‑1 human acute monocytic leukemia cell line (untreated; negative staining) using Mouse Anti-Human CXCL9/MIG Monoclonal Antibody (Catalog # MAB392) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Chemotaxis Induced by CXCL9/MIG and Neutralization by Human CXCL9/MIG Antibody. View Larger

Chemotaxis Induced by CXCL9/MIG and Neutralization by Human CXCL9/MIG Antibody. Recombinant Human CXCL9/MIG (Catalog # 392-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL9/MIG (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL9/MIG Monoclonal Antibody (Catalog # MAB392). The ND50 is typically 0.5-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL9/MIG

CXCL9, a member of the alpha  subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide that is cleaved to yield a 103 aa residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic aa residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. The E. coli-expressed CXCL9 preparations produced at R&D Systems have been shown to contain greater than 80% full length CXCL9.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Liao, F. et al. (1995) J. Exp. Med. 182:1301.
  3. Vanguri, P. (1995) J. Neuroimmunol. 56:35.
Entrez Gene IDs
4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma

Product Datasheets

Citations for Human CXCL9/MIG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy
    Authors: M Pascual-Ga, E Bonfill-Te, E Planas-Rig, C Rubio-Pere, R Iurlaro, A Arias, I Cuartas, A Sala-Hojma, L Escudero, F Martínez-R, I Huber-Ruan, P Nuciforo, L Pedrosa, C Marques, I Braña, E Garralda, M Vieito, M Squatrito, E Pineda, F Graus, C Espejo, J Sahuquillo, J Tabernero, J Seoane
    Nat Commun, 2019;10(1):2416.
    Species: Human
    Sample Types: Organoid
    Applications: Neutralization
  2. CXCL9 and CXCL10 predict survival and are regulated by cyclooxygenase inhibition in advanced serous ovarian cancer
    Br J Cancer, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014;14(0):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
    Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
    J. Invest. Dermatol., 2011;131(1):133-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
    Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
    Authors: Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA
    Eur. Respir. J., 2009;34(3):676-86.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  10. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
    Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
    J. Immunol., 2007;179(6):4272-82.
    Species: Human
    Sample Types: Serum
    Applications: Immunodepletion
  11. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  12. Up-regulation of the interferon gamma (IFN-gamma)-inducible chemokines IFN-inducible T-cell alpha chemoattractant and monokine induced by IFN-gamma and of their receptor CXC receptor 3 in human renal cell carcinoma.
    Authors: Suyama T, Furuya M, Nishiyama M, Kasuya Y, Kimura S, Ichikawa T, Ueda T, Nikaido T, Ito H, Ishikura H
    Cancer, 2005;103(2):258-67.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow.
    Authors: Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH
    Am. J. Pathol., 2005;167(3):887-99.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human CXCL9/MIG Antibody

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Human CXCL9/MIG Antibody
By Anonymous on 09/04/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: macrophages Species: Human

CXCL9 antibody showed positive macrophages in hepatitis liver tissue.


Human CXCL9/MIG Antibody
By Anonymous on 12/06/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as a capture in a sandwich ELISA for MIG. The detection antibody was BAM392. The standard was 392-MG-010. Sensitivity was around 5 pg/ml.