Human CXCR3 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB160A-025
FAB160A-100
Detection of CXCR3 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (2)
FAQs
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Reviews (1)

Human CXCR3 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCR3.
Source
Monoclonal Mouse IgG1 Clone # 49801
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR3
Met1-Leu368
Accession # P49682
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CXCR3 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CXCR3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR3 APC-conjugated Monoclonal Antibody (Catalog # FAB160A) and Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR3

CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

Entrez Gene IDs
2833 (Human); 12766 (Mouse); 84475 (Rat)
Alternate Names
CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR

Product Datasheets

Citations for Human CXCR3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Detection of Primary T Cell Responses to Drugs and Chemicals in HLA-Typed Volunteers: Implications for the Prediction of Drug Immunogenicity
    Authors: Lee Faulkner
    Toxicol. Sci, 2016;154(2):416-429.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Intravenous immunoglobulins suppress T-cell priming by modulating the bidirectional interaction between dendritic cells and natural killer cells.
    Authors: Tha-In T, Metselaar HJ, Tilanus HW, Groothuismink ZM, Kuipers EJ, De Man RA, Kwekkeboom J
    Blood, 2007;110(9):3253-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CXCR3 APC-conjugated Antibody

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Human CXCR3 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Cytometry Sample Tested: Peripheral blood cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100