Human CXCR3 Antibody

(40 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCR3.
  • Source
    Monoclonal Mouse IgG1 Clone # 49801
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CXCR3
    Met1-Leu368
    Accession # P49682
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood lymphocytes
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 7 ng/mL Recombinant Human CXCL11/I‑TAC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCR3 Antibody. Recombinant Human CXCL11/I‑TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (7 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160). The ND50 is typically 0.3-1.5 µg/mL.
Immunohistochemistry
CXCR3 in Human Tonsil. CXCR3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Human CXCR3 Monoclonal Antibody (Catalog # MAB160) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR3

CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

  • Entrez Gene IDs:
    2833 (Human); 12766 (Mouse); 84475 (Rat)
  • Alternate Names:
    CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages
    Authors: F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence
    Authors: N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra
    Nat Commun, 2017;8(0):15380.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  3. Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells
    Authors: H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt
    Oncol Lett, 2017;13(6):4224-4230.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. CXCR3 mediates ascites-directed tumor cell migration and predicts poor outcome in ovarian cancer patients
    Authors: C Windmüller, D Zech, S Avril, M Boxberg, T Dawidek, B Schmalfeld, M Schmitt, M Kiechle, H Bronger
    Oncogenesis, 2017;6(5):e331.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  5. Elevated circulating levels of the interferon-?-induced chemokines are associated with disease activity and cutaneous manifestations in adult-onset Still's disease
    Authors: JH Han, CH Suh, JY Jung, MH Ahn, MH Han, JE Kwon, H Yim, HA Kim
    Sci Rep, 2017;7(0):46652.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  6. Skewing towards Treg and Th2 responses is a characteristic feature of sustained remission in ANCA-positive granulomatosis with polyangiitis
    Authors: W Szczeklik, B Jakie?a, K Wawrzycka-, M Sanak, M Hubalewska, A Padjas, M Surmiak, K Szczeklik, J Sznajd, J Musia?
    Eur. J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. TNF-? augments CXCR2 and CXCR3 to promote progression of renal cell carcinoma
    Authors: Kuang-Hui Sun
    J Cell Mol Med, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
    Authors: Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G
    Clin Cancer Res, 2015;21(12):2840-50.
  9. CXCR3 Polymorphism and Expression Associate with Spontaneous Preterm Birth.
    Authors: Karjalainen M, Ojaniemi M, Haapalainen A, Mahlman M, Salminen A, Huusko J, Maatta T, Kaukola T, Anttonen J, Ulvila J, Haataja R, Teramo K, Kingsmore S, Palotie A, Muglia L, Ramet M, Hallman M
    J Immunol, 2015;195(5):2187-98.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  10. Skin Metabolites Define a New Paradigm in the Localization of Skin Tropic Memory T Cells.
    Authors: McCully M, Collins P, Hughes T, Thomas C, Billen J, O'Donnell V, Moser B
    J Immunol, 2015;195(1):96-104.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Dysregulation of CXCR3 expression on peripheral blood leukocytes in patients with neovascular age-related macular degeneration.
    Authors: Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T
    Invest Ophthalmol Vis Sci, 2014;55(7):4050-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
    Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
    J Immunol, 2014;192(8):3805-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Role of CXCR3/CXCL10 axis in immune cell recruitment into the small intestine in celiac disease.
    Authors: Bondar C, Araya R, Guzman L, Rua E, Chopita N, Chirdo F
    PLoS ONE, 2014;9(2):e89068.
    Species: Human
    Sample Type: Whole Tissue
    Application: IF
  14. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014;14(0):118.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fresh
  15. Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis.
    Authors: Chaturvedi, Pallavi, Gilkes, Daniele, Wong, Carmen C, Luo, Weibo, Zhang, Huafeng, Wei, Hong, Takano, Naoharu, Schito, Luana, Levchenko, Andre, Semenza, Gregg L
    J Clin Invest, 2013;123(1):189-205.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. Human trophectoderm apposition is regulated by interferon gamma-induced protein 10 (IP-10) during early implantation.
    Authors: Sela H, Goldman-Wohl D, Haimov-Kochman R, Greenfield C, Natanson-Yaron S, Hamani Y, Revel A, Lavy Y, Singer O, Yachimovich-Cohen N, Turetsky T, Mandelboim O, Reubinoff B, Yagel S
    Placenta, 2013;34(3):222-30.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  17. Neuroblastoma-derived TGF-beta1 modulates the chemokine receptor repertoire of human resting NK cells.
    Authors: Castriconi R, Dondero A, Bellora F, Moretta L, Castellano A, Locatelli F, Corrias M, Moretta A, Bottino C
    J Immunol, 2013;190(10):5321-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Long-term Vaccination with Multiple Peptides Derived from Cancer-Testis Antigens Can Maintain a Specific T-cell Response and Achieve Disease Stability in Advanced Biliary Tract Cancer.
    Authors: Aruga A, Takeshita N, Kotera Y, Okuyama R, Matsushita N, Ohta T, Takeda K, Yamamoto M
    Clin Cancer Res, 2013;19(8):2224-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. Altered CXCR3 isoform expression regulates prostate cancer cell migration and invasion.
    Authors: Wu Q, Dhir R, Wells A
    Mol. Cancer, 2012;11(0):3.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  20. Ubiquitination of CXCR7 controls receptor trafficking.
    Authors: Canals M, Scholten DJ, de Munnik S, Han MK, Smit MJ, Leurs R
    PLoS ONE, 2012;7(3):e34192.
    Species: Human
    Sample Type: Whole Cells
    Application: Internalization Assay
  21. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  22. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
    Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
    J. Immunol., 2009;182(6):3919-27.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  23. CXCR3 activation by lentivirus infection suppresses neuronal autophagy: neuroprotective effects of antiretroviral therapy.
    Authors: Zhu Y, Vergote D, Pardo C, Noorbakhsh F, McArthur JC, Hollenberg MD, Overall CM, Power C
    FASEB J., 2009;23(9):2928-41.
    Species: Feline
    Sample Type: Tissue Homogenates
    Application: Functional Assay
  24. CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
    Authors: Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA
    Eur. Respir. J., 2009;34(3):676-86.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  25. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  26. The chemokine receptor CXCR3 is degraded following internalization and is replenished at the cell surface by de novo synthesis of receptor.
    Authors: Meiser A, Mueller A, Wise EL, McDonagh EM, Petit SJ, Saran N, Clark PC, Williams TJ, Pease JE
    J. Immunol., 2008;180(10):6713-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  27. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  28. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007;179(6):3724-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  29. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
    Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
    J. Immunol., 2007;179(6):4272-82.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  30. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  31. Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma.
    Authors: Srivastava BI, Srivastava MD
    Leuk. Res., 2005;29(7):771-83.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  32. Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
    Authors: Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B
    Arthritis Rheum., 2005;52(5):1504-16.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Fresh/Frozen
  33. Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11.
    Authors: Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B
    J. Neuroimmunol., 2005;163(1):128-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  34. Chemokine receptor expression in rat adjuvant-induced arthritis.
    Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE
    Arthritis Rheum., 2005;52(12):3718-30.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  35. Up-regulation of the interferon gamma (IFN-gamma)-inducible chemokines IFN-inducible T-cell alpha chemoattractant and monokine induced by IFN-gamma and of their receptor CXC receptor 3 in human renal cell carcinoma.
    Authors: Suyama T, Furuya M, Nishiyama M, Kasuya Y, Kimura S, Ichikawa T, Ueda T, Nikaido T, Ito H, Ishikura H
    Cancer, 2005;103(2):258-67.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  36. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004;114(11):1640-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  37. Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
    Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
    Int. J. Oncol., 2004;25(3):605-13.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  38. Human immunodeficiency virus type 1 Nef protein mediates neural cell death: a neurotoxic role for IP-10.
    Authors: van Marle G, Henry S, Todoruk T, Sullivan A, Silva C, Rourke SB, Holden J, McArthur JC, Gill MJ, Power C
    Virology, 2004;329(2):302-18.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  39. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002;168(12):6244-52.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  40. Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
    Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
    Eur. J. Immunol., 2001;31(4):1037-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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