Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Feline, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, Cell Culture, ELISA Detection, Functional Assay, Stimulation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 49801
View all CXCR3 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR3
Met1-Leu368
Accession # P49682

Specificity

Detects human CXCR3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCR3 Antibody

Detection of CXCR3 antibody in Human PBMC Lymphocytes antibody by Flow Cytometry.

Detection of CXCR3 in Human PBMC Lymphocytes by Flow Cytometry.

Human PBMC lymphocytes were stained with (A) Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160) or (B) Mouse IgG1 isotype control antibody (MAB002) followed by PE-conjugated anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (FAB100A).View our protocol for Staining Membrane-associated Proteins.
Detection of CXCR3 antibody in HEK293 Human Cell Line Transfected with human CXCR3 and eGFP antibody by Flow Cytometry.

Detection of CXCR3 in HEK293 Human Cell Line Transfected with human CXCR3 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human CXCR3 or (B) irrelevant transfectants and eGFP was stained with and either Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG1 Flow Cytometry Isotype Control (MAB002). View our protocol for Staining Membrane-associated Proteins.
CXCR3 antibody in Human Tonsil by Immunohistochemistry (IHC-P).

CXCR3 in Human Tonsil.

CXCR3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Human CXCR3 Monoclonal Antibody (Catalog # MAB160) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCR3 Antibody.

Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCR3 Antibody.

Recombinant Human CXCL11/I-TAC (672-IT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL11/I-TAC (7 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160). The ND50 is typically 0.3-1.5 µg/mL.
Detection of Human CXCR3 by Western Blot

Detection of Human CXCR3 by Western Blot

Functional effects of IP-10 on PCCs(A) Expression of CXCR3 in PCCs, PSCs and HEK293-EBNA/CXCR3-B analyzed by Western Blot. alpha -tubulin was used as loading control. (B) Microphotographs of PCCs migrated through the Boyden chamber in response to 0.5% FBS or IP-10 (24 hr). (C-D) Viability of PCCs after IP-10 addition determined using the MTT assay. Low/high refers to cell seeding density. Data are representative of two/three independent experiments. Data in the histograms are presented as mean ± SD. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.2519), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CXCR3 by Immunocytochemistry/Immunofluorescence

Detection of Human CXCR3 by Immunocytochemistry/Immunofluorescence

Infiltration of CXCR3+ cells in the lamina propria of small intestine mucosa.a. Confocal immunofluorescence for CXCR3 was performed in sections of duodenal biopsies from controls (i), untreated celiac patients (ii), and treated celiac patients (iii). CXCR3 is shown in green, and nuclei are shown in red. Untreated celiac patients showed a higher number of positive cells infiltrating the LP. (Magnification, 630×). b. The number of CXCR3+ cells in the LP was higher in the duodenal mucosa of untreated celiac patients (n = 9) compared with control individuals (n = 6) and treated patients (n = 5) (unpaired t-test; p = 0.0089 and p = 0.0055, respectively). The positive cells in LP regions from sections of duodenal biopsies were counted using immunofluorescence microscopy. c. Representative flow cytometric analysis from the LP compartment of a duodenal sample of an untreated CD patient showing plasma cells (CD138+) that express CXCR3. d. Representative flow cytometric analysis from the LP compartment of a duodenal sample of an untreated CD patient showing LP lymphocytes (CD3+ or CD4+) that express CXCR3. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0089068), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC lymphocytes and HEK293 human cell line transfected with human CXCR3 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Neutralization

Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 7 ng/mL Recombinant Human CXCL11/I‑TAC.

Reviewed Applications

Read 2 reviews rated 5 using MAB160 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR3

CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

Alternate Names

CD183, CXCR3, GPR9

Entrez Gene IDs

2833 (Human); 12766 (Mouse); 84475 (Rat)

Gene Symbol

CXCR3

UniProt

P49682

Additional CXCR3 Products

Product Documents for Human CXCR3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCR3 Antibody

For research use only

Citations for Human CXCR3 Antibody

Customer Reviews for Human CXCR3 Antibody (2)

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  • Human CXCR3 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 01/24/2021
    Human CXCR3 Antibody MAB160
  • Human CXCR3 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: J45.01 human acute lymphoblastic leukemia T lymphocyte cell line
    Species: Human
    Verified Customer | Posted 11/15/2018
    Human CXCR3 Antibody MAB160

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Protocols

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