Human CXCR3 Fluorescein-conjugated Antibody

(21 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCR3.
  • Source
    Monoclonal Mouse IgG1 Clone # 49801
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CXCR3
    Met1-Leu368
    Accession # P49682
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB160F) and Mouse Anti-Human CD3 epsilon Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB100N). Quadrant markers were set based on control antibody staining (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR3

CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

  • Entrez Gene IDs:
    2833 (Human); 12766 (Mouse); 84475 (Rat)
  • Alternate Names:
    CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. A distinct plasmablast and na�ve B-cell phenotype in primary immune thrombocytopenia
    Haematologica, 2016;101(6):698-706.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
    Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
    J Immunol, 2014;192(12):5776-88.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Phase I clinical trial of multiple-peptide vaccination for patients with advanced biliary tract cancer.
    Authors: Aruga, Atsushi, Takeshita, Nobuhiro, Kotera, Yoshihit, Okuyama, Ryuji, Matsushita, Norimasa, Ohta, Takehiro, Takeda, Kazuyosh, Yamamoto, Masakazu
    J Transl Med, 2014;12(0):61.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation.
    Authors: Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H
    J Immunol, 2013;190(7):3696-705.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Plasmablasts as migratory IgG-producing cells in the pathogenesis of neuromyelitis optica.
    Authors: Chihara, Norio, Aranami, Toshimas, Oki, Shinji, Matsuoka, Takako, Nakamura, Masakazu, Kishida, Hitaru, Yokoyama, Kazumasa, Kuroiwa, Yoshiyuk, Hattori, Nobutaka, Okamoto, Tomoko, Murata, Miho, Toda, Tatsushi, Miyake, Sachiko, Yamamura, Takashi
    PLoS ONE, 2013;8(12):e83036.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Human TCR gamma delta+ T cells represent a novel target for IL-27 activity.
    Authors: Morandi F, Prigione I, Airoldi I
    Eur. J. Immunol., 2012;42(6):1547-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Biomarkers on melanoma patient T cells associated with ipilimumab treatment.
    Authors: Wang W, Yu D, Sarnaik A, Yu B, Hall M, Morelli D, Zhang Y, Zhao X, Weber J
    J Transl Med, 2012;10(0):146.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
    Authors: Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM
    J. Immunol., 2010;185(11):6646-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. CXCR3-B can mediate growth-inhibitory signals in human renal cancer cells by down-regulating the expression of heme oxygenase-1.
    Authors: Datta D, Banerjee P, Gasser M
    J. Biol. Chem., 2010;285(47):36842-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Phenotypic and functional markers for 1alpha,25-dihydroxyvitamin D(3)-modified regulatory dendritic cells.
    Authors: Pedersen AW, Holmstrom K, Jensen SS, Fuchs D, Rasmussen S, Kvistborg P, Claesson MH, Zocca MB
    Clin. Exp. Immunol., 2010;157(1):48-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. The chemokine receptor CXCR3 is degraded following internalization and is replenished at the cell surface by de novo synthesis of receptor.
    Authors: Meiser A, Mueller A, Wise EL, McDonagh EM, Petit SJ, Saran N, Clark PC, Williams TJ, Pease JE
    J. Immunol., 2008;180(10):6713-24.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007;82(4):946-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
    Authors: Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V
    Allergy, 2007;62(2):170-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis.
    Authors: Mamoni RL, Blotta MH
    Cytokine, 2006;35(3):207-16.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow
  18. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004;112(2):274-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
    Authors: D'Ambrosio D
    Eur. J. Immunol., 2002;32(10):2934-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
    Authors: Janatpour MJ, Hudak S, Sathe M, McEvoy LM
    J. Exp. Med., 2001;194(9):1375-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 21 Citations
Isotype Controls
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Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 14  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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