Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Cystatin B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cystatin B (R&D Systems, Catalog # 1408-PI)
Met2-Phe98
Accession # P04080

Specificity

Detects human Cystatin B in Western blots. In this format, less than 2% cross-reactivity with recombinant mouse Cystatin B, recombinant human (rh) Cystatin A, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin S, rhCystatin SA, and rhCystatin SN is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Cystatin B Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cystatin B (Catalog # 1408-PI)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cystatin B

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Mutations in the Cystatin B gene is the cause of progressive myoclonus epilepsy (EPM1) (3). Because of its expression patterns, Cystatin B can be used as a marker for certain cancers, such as glioblastoma tumors (4). It readily forms amyloid fibrils in vitro (5). The human Cystatin B consists of 98 amino acid residues (3).

References

  1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  2. Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
  3. Pennacchio, L.A. et al. (1996) Science 271:1731.
  4. Zhang, R. et al. (2003) Glia 42:194.
  5. Zerovnik, E. et al. (2002) Biochim. Biophys. Acta 1594:1.

Alternate Names

CSTB, Stefin B

Entrez Gene IDs

1476 (Human); 13014 (Mouse)

Gene Symbol

CSTB

UniProt

P04080

Additional Cystatin B Products

Product Documents for Human Cystatin B Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cystatin B Biotinylated Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

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