Human Cytokeratin 5 Antibody

Name: Human Cytokeratin 5 Antibody
Brand: R&D Systems
SKU: AF7564

Catalog #: AF7564 Datasheet / COA / SDS

Discontinued Product

AF7564 has been discontinued.

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Detection of Human Cytokeratin 5 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cytokeratin 5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cytokeratin 5 in direct ELISA and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) KRT-14, rhKRT-18, rhKRT-19, and rhKRT36 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Cytokeratin 5
Met1-Gly98
Accession # P13647

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Cytokeratin 5 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Cytokeratin 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7564) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytokeratin 5 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background:

Cytokeratin 5 (Keratin, type II cytoskeletal 5; also 58 kDa cytokeratin, CK-5 and Keratin-5) is a 58-59 kDa Class II (large keratins of basic pH) member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class II KRT-5 heterodimerizes with 50-52 kDa class I KRT-14 to form 8 nm filaments that likely serve as a cytoskeletal scaffold. Both keratin-5 and -14 are also suggested to positively regulate keratinocyte cell cycle progression. Both keratins are associated with cells of sweat gland ducts and the basale layer cells of both keratinized stratified squamous plus transitional epithelium. Human cytokeratin 5 is 590 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-167), a subsequent "rod" region (aa 168-477) with two coiled segments, and a C-terminal tail region that is rich in serine (aa 478-590). Cytokeratin 5 possesses at least three utilized phosphorylation sites at Tyr60, Tyr 66 and Ser75. There are at least three potential isoform variants. One possesses a 26 aa substitution for aa 527-590, a second shows a deletion of aa 71-93, and a third contains an 11 aa substitution for aa 349-590. Over aa 1-98, human cytokeratin 5 shares 83% aa sequence identity with mouse cytokeratin 5.