Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 406209
View all DAP12 Primary Antibodies »

Product Specifications

Immunogen

Human Dap12 synthetic peptide
Accession # O43914

Specificity

Detects human DAP12 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human DAP12 Antibody

Detection of DAP12 in Human CD56+Natural Killer Cells by Flow Cytometry.

Human PBMCs were stained with (A) Human DAP12 Monoclonal Antibody (Catalog # MAB52401) or (B) isotype control antibody (MAB003), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B) and Rabbit anti-human CD56 PE-conjugated Monoclonal Antibody (FAB24086P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (1X) (FC005).

Applications for Human DAP12 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/mL
Sample: Human PBMCs

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DAP12

DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.

Long Name

DNAX-activation Protein 12

Alternate Names

KARAP, TYROBP

Entrez Gene IDs

7305 (Human); 22177 (Mouse); 361537 (Rat)

Gene Symbol

TYROBP

UniProt

O43914 (Human)

Additional DAP12 Products

Product Documents for Human DAP12 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DAP12 Antibody

For research use only

Citations for Human DAP12 Antibody

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