Human DAP12 Antibody

Catalog # Availability Size / Price Qty
Detection of DAP12 in Human CD56+Natural Killer Cells by Flow Cytometry.
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Product Details

Human DAP12 Antibody Summary

Detects human DAP12 in Western blots.
Monoclonal Mouse IgG2A Clone # 406209
Protein A or G purified from hybridoma culture supernatant
Human Dap12 synthetic peptide
Accession # O43914
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Scientific Data

Intracellular Staining by Flow Cytometry View Larger

Detection of DAP12 in Human CD56+Natural Killer Cells by Flow Cytometry. Human PBMCs were stained with (A) Human DAP12 Monoclonal Antibody (Catalog # MAB52401) or (B) isotype control antibody (MAB003), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B) and Rabbit anti-human CD56 PE-conjugated Monoclonal Antibody (FAB24086P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (1X) (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (1X) (FC005).

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAP12

DAP12, also known as TYROBP and KARAP, is a transmembrane protein that functions as a signal transduction adaptor molecule. DAP12 is expressed as a disulfide-linked homodimer that associates with a variety of receptors on NK and myeloid cells. Complex formation is mediated by intramembrane ionic interaction between an aspartic acid residue in DAP12 and a lysine residue in the partnered receptor. Ligation of these receptors can trigger either cell activation or inhibition through the ITAM sequence in DAP12, resulting in activation of Src family tyrosine kinases. Human and mouse DAP12 share 73% amino acid sequence identity.

Long Name
DNAX-activation Protein 12
Entrez Gene IDs
7305 (Human); 22177 (Mouse); 361537 (Rat)
Alternate Names
DAP12; DAP12PLOSL; DNAX-activation protein 12; KARAP; KARAPPLO-SL; KAR-associated protein; killer activating receptor associated protein; Killer-activating receptor-associated protein; TYRO protein tyrosine kinase binding protein; TYRO protein tyrosine kinase-binding protein; TYROBP

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