Human DC-SIGN/CD209 Fluorescein-conjugated Antibody

Clone 120507 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB161F-100
FAB161F-025
Human DC-SIGN/CD209 Fluorescein-conjugated Antibody in Data
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Citations (10)
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Human DC-SIGN/CD209 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
Source
Monoclonal Mouse IgG2B Clone # 120507
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of DC‑SIGN/CD209 in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 by Flow Cytometry. View Larger

Detection of DC‑SIGN/CD209 in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGN/CD209 was stained with Mouse Anti-Human DC-SIGN/CD209 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB161F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-SIGN/CD209

Human DC-Sign (dendritic cell-specific ICAM-3 grabbing nonintegrin; also known as CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Su, S.V. et al. (2004) J. Biol. Chem. 279:19122.
  5. Cambi, A. et al. (2005) Cell. Microbiol. 7:481.
  6. Serrano-Gomez, D. et al. (2004) J. Immunol. 173:5635.
  7. Geijtenbeek, T.B.H. and Y. van Kooyk (2003) Curr. Top. Microbiol. Immunol. 276:32.
  8. Baribaud, F. et al. (2001) J. Virol. 75:10281.
  9. Wu, L. et al. (2002) J. Virol. 76:5905.
  10. Baribaud, F. et al. (2002) J. Virol.76:9135.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs
30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)
Alternate Names
CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Product Datasheets

Citations for Human DC-SIGN/CD209 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. HIV-1 exposure and immune activation enhance sexual transmission of Hepatitis C virus by primary Langerhans cells
    Authors: BM Nijmeijer, R Sarrami-Fo, GS Steba, RR Schreurs, SM Koekkoek, R Molenkamp, J Schinkel, P Reiss, ML Siegenbeek, M van der Va, CM Ribeiro, TB Geijtenbee
    J Int AIDS Soc, 2019;22(3):e25268.
    Species: Human
    Sample Types: human
    Applications: Flow Cytometry
  2. Differentiation of Langerhans Cells from Monocytes and Their Specific Function in Inducing IL-22-Specific Th Cells
    Authors: Y Otsuka, E Watanabe, E Shinya, S Okura, H Saeki, TBH Geijtenbee, H Takahashi
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. DCs facilitate B cell responses against microbial DNA via DC-SIGN
    Authors: JK Sprokholt, MH Heineke, TM Kaptein, JL van Hamme, TBH Geijtenbee
    PLoS ONE, 2017;12(10):e0185580.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Dendritic cells from the human female reproductive tract rapidly capture and respond to HIV
    Mucosal Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Langerhans Cell-Dendritic Cell Cross-Talk via Langerin and Hyaluronic Acid Mediates Antigen Transfer and Cross-Presentation of HIV-1.
    Authors: van den Berg L, Cardinaud S, van der Aar A, Sprokholt J, de Jong M, Zijlstra-Willems E, Moris A, Geijtenbeek T
    J Immunol, 2015;195(4):1763-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Antibody-opsonized bacteria evoke an inflammatory dendritic cell phenotype and polyfunctional Th cells by cross-talk between TLRs and FcRs.
    Authors: Bakema J, Tuk C, van Vliet S, Bruijns S, Vos J, Letsiou S, Dijkstra C, van Kooyk Y, Brenkman A, van Egmond M
    J Immunol, 2015;194(4):1856-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Protective effects of astaxanthin on ConA-induced autoimmune hepatitis by the JNK/p-JNK pathway-mediated inhibition of autophagy and apoptosis.
    Authors: Li J, Xia Y, Liu T, Wang J, Dai W, Wang F, Zheng Y, Chen K, Li S, Abudumijiti H, Zhou Z, Wang J, Lu W, Zhu R, Yang J, Zhang H, Yin Q, Wang C, Zhou Y, Lu J, Zhou Y, Guo C
    PLoS ONE, 2015;10(3):e0120440.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Epitope specificity and clonality of EBV-specific CTLs used to treat posttransplant lymphoproliferative disease.
    Authors: McAulay KA, Haque T, Urquhart G, Bellamy C, Guiretti D, Crawford DH
    J. Immunol., 2009;182(6):3892-901.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
    Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(47):16606-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Involvement of macrophage mannose receptor in the binding and transmission of HIV by macrophages.
    Authors: Nguyen DG, Hildreth JE
    Eur. J. Immunol., 2003;33(2):483-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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