Human DC-SIGN/CD209 Antibody

Clone 120507 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB161-SP
MAB161-100
MAB161-500
Human DC-SIGN/CD209 Antibody in Flow Cytometry
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Product Details
Citations (36)
FAQs
Supplemental Products
Reviews (1)

Human DC-SIGN/CD209 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
Source
Monoclonal Mouse IgG2B Clone # 120507
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human DC-SIGN Fc Chimera (Catalog # 161-DC)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human lymph node
Adhesion Blockade
The adhesion of NIH-3T3 mouse embryonic fibroblast cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of DC‑SIGN in Human DC‑SIGN Transfected 3T3 Mouse Cell Line by Flow Cytometry. View Larger

Detection of DC‑SIGN in Human DC‑SIGN Transfected 3T3 Mouse Cell Line by Flow Cytometry. Human DC-SIGN and DC-SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of DC‑SIGN in Human Monocyte Derived Dendritic Cells by Flow Cytometry. View Larger

Detection of DC‑SIGN in Human Monocyte Derived Dendritic Cells by Flow Cytometry. Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Immunocytochemistry DC-SIGN in Human Dendritic Cells. View Larger

DC-SIGN in Human Dendritic Cells. DC-SIGN was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-SIGN/CD209

Human DC-Sign (dendritic cell-specific ICAM-3 grabbing nonintegrin; also CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Su, S.V. et al. (2004) J. Biol. Chem. 279:19122.
  5. Cambi, A. et al. (2005) Cell. Microbiol. 7:481.
  6. Serrano-Gomez, D. et al. (2004) J. Immunol. 173:5635.
  7. Geijtenbeek, T.B.H. and Y. van Kooyk (2003) Curr. Top. Microbiol. Immunol. 276:32.
  8. Baribaud, F. et al. (2001) J. Virol. 75:10281.
  9. Wu, L. et al. (2002) J. Virol. 76:5905.
  10. Baribaud, F. et al. (2002) J. Virol.76:9135.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs
30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)
Alternate Names
CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Product Datasheets

Citations for Human DC-SIGN/CD209 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Cell-to-Cell Spreading of HIV-1 in Myeloid Target Cells Escapes SAMHD1 Restriction
    Authors: M Xie, H Leroy, R Mascarau, M Woottum, M Dupont, C Ciccone, A Schmitt, B Raynaud-Me, C Vérollet, J Bouchet, L Bracq, S Benichou
    MBio, 2019;10(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Dengue viruses infect human megakaryocytes, with probable clinical consequences
    Authors: MB Vogt, A Lahon, RP Arya, JL Spencer Cl, R Rico-Hesse
    PLoS Negl Trop Dis, 2019;13(11):e0007837.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Tricarboxylic Acid Cycle Activity and Remodeling of Glycerophosphocholine Lipids Support Cytokine Induction in Response to Fungal Patterns
    Authors: S Márquez, JJ Fernández, C Mancebo, C Herrero-Sá, S Alonso, TA Sandoval, M Rodríguez, JR Cubillos-R, O Montero, N Fernández, M Sánchez Cr
    Cell Rep, 2019;27(2):525-536.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-?
    Authors: KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten
    Eur. J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Metformin Uniquely Prevents Thrombosis by Inhibiting Platelet Activation and mtDNA Release
    Sci Rep, 2016;6(0):36222.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
    PLoS ONE, 2016;11(11):e0167410.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
    Sci Rep, 2016;6(0):29447.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. Clearance of autophagy-associated dying retinal pigment epithelial cells - a possible source for inflammation in age-related macular degeneration
    Cell Death Dis, 2016;7(9):e2367.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk.
    Authors: El-Awady, Ahmed R, Miles, Brodie, Scisci, Elizabet, Kurago, Zoya B, Palani, Chithra, Arce, Roger M, Waller, Jennifer, Genco, Caroline, Slocum, Connie, Manning, Matthew, Schoenlein, Patricia, Cutler, Christop
    PLoS Pathog, 2015;10(2):e1004647.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
    Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
    PLoS ONE, 2015;10(3):e0120948.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  11. Live-attenuated measles virus vaccine targets dendritic cells and macrophages in muscle of nonhuman primates.
    Authors: Rennick L, de Vries R, Carsillo T, Lemon K, van Amerongen G, Ludlow M, Nguyen D, Yuksel S, Verburgh R, Haddock P, McQuaid S, Duprex W, de Swart R
    J Virol, 2015;89(4):2192-200.
  12. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
    Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K
    PLoS Pathog, 2014;10(12):e1004548.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  13. In vivo targeting of human DC-SIGN drastically enhances CD8(+) T-cell-mediated protective immunity.
    Authors: Hesse C, Ginter W, Forg T, Mayer C, Baru A, Arnold-Schrauf C, Unger W, Kalay H, van Kooyk Y, Berod L, Sparwasser T
    Eur J Immunol, 2014;43(10):2543-53.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Early biodistribution and persistence of a protective live attenuated SIV vaccine elicits localised innate responses in multiple lymphoid tissues.
    Authors: Ferguson D, Mattiuzzo G, Ham C, Stebbings R, Li B, Rose N, Mee E, Smith D, Page M, Cranage M, Almond N, Towers G, Berry N
    PLoS ONE, 2014;9(8):e104390.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients.
    Authors: Beck, Zoltan, Jagodzinski, Linda L, Eller, Michael, Thelian, Doris, Matyas, Gary R, Kunz, Anjali N, Alving, Carl R
    PLoS ONE, 2013;8(11):e81002.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
    Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
    Blood, 2013;121(1):95-106.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
    Authors: Johnson TR, McLellan JS, Graham BS
    J. Virol., 2012;86(3):1339-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.
    Authors: Emara M, Royer PJ, Mahdavi J
    J. Biol. Chem., 2012;287(8):5756-63.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  19. In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa.
    Authors: Hirbod T, Kaldensjo T, Broliden K
    PLoS ONE, 2011;6(9):e25551.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Semen clusterin is a novel DC-SIGN ligand.
    Authors: Sabatte J, Faigle W, Ceballos A, Morelle W, Rodrígues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
    J. Immunol., 2011;187(10):5299-309.
    Species: Human
    Sample Types: Seminal Plasma
    Applications: Flow Cytometry
  21. Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations.
    Authors: Bond E, Liang F, Sandgren KJ, Smed-Sorensen A, Bergman P, Brighenti S, Adams WC, Betemariam SA, Rangaka MX, Lange C, Wilkinson RJ, Andersson J, Lore K
    J. Invest. Dermatol., 2011;132(1):114-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells.
    Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B
    J. Virol., 2009;83(21):10908-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation.
    Authors: Ogawa Y, Kawamura T, Kimura T, Ito M, Blauvelt A, Shimada S
    Blood, 2009;113(21):5157-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells.
    Authors: Nonaka M, Ma BY, Murai R, Nakamura N, Baba M, Kawasaki N, Hodohara K, Asano S, Kawasaki T
    J. Immunol., 2008;180(5):3347-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
    Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
    Mucosal Immunol, 2008;1(5):412-20.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli.
    Authors: Iyori M, Ohtani M, Hasebe A, Totsuka Y, Shibata K
    Biochem. Biophys. Res. Commun., 2008;377(2):367-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response.
    Authors: Wang J, Zhang Y, Wei J, Zhang X, Zhang B, Zhu Z, Zou W, Wang Y, Mou Z, Ni B, Wu Y
    Immunology, 2007;121(2):174-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles.
    Authors: Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH
    Am. J. Pathol., 2006;169(1):200-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  29. Recombinant vesicular stomatitis viruses encoding simian immunodeficiency virus receptors target infected cells and control infection.
    Authors: Okuma K, Boritz E, Walker J, Sarkar A, Alexander L, Rose JK
    Virology, 2006;346(1):86-97.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. BCR/ABL promotes dendritic cell-mediated natural killer cell activation.
    Authors: Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L
    Cancer Res., 2005;65(14):6409-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. DC-SIGN-mediated infectious synapse formation enhances X4 HIV-1 transmission from dendritic cells to T cells.
    Authors: Arrighi JF, Pion M, Garcia E, Escola JM, van Kooyk Y, Geijtenbeek TB, Piguet V
    J. Exp. Med., 2004;200(10):1279-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Urine dendritic cells: a noninvasive probe for immune activity in bladder cancer?
    Authors: Beatty JD, Islam S, North ME, Knight SC, Ogden CW
    BJU Int., 2004;94(9):1377-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
    Authors: Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L
    Blood, 2004;104(10):3267-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. DC-SIGN is the major Mycobacterium tuberculosis receptor on human dendritic cells.
    Authors: Tailleux L, Schwartz O, Herrmann JL, Pivert E, Jackson M, Amara A, Legres L, Dreher D, Nicod LP, Gluckman JC, Lagrange PH, Gicquel B, Neyrolles O
    J. Exp. Med., 2003;197(1):121-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. Dendritic cells in the human decidua.
    Authors: Gardner L, Moffett A
    Biol. Reprod., 2003;69(4):1438-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  36. Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN.
    Authors: Wu L, Bashirova AA, Martin TD, Villamide L, Mehlhop E, Chertov AO, Unutmaz D, Pope M, Carrington M, KewalRamani VN
    Proc. Natl. Acad. Sci. U.S.A., 2002;99(3):1568-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human DC-SIGN/CD209 Antibody
By Anonymous on 07/27/2016
Application: Flow Sample Tested: Dendritic cells Species: Human