Human DC-STAMP APC‑conjugated Antibody

R&D Systems | Catalog # FAB7824A

R&D Systems
Discontinued Product
FAB7824A has been discontinued. View all DC-STAMP products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 788524
View all DC-STAMP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DC-STAMP
Asp314-Thr376
Accession # Q9H295

Specificity

Detects human DC-STAMP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse DC-STAMP is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DC-STAMP APC‑conjugated Antibody

Detection of DC-STAMP antibody in Human Denditic Cells antibody by Flow Cytometry.

Detection of DC-STAMP in Human Denditic Cells by Flow Cytometry.

Mature human monocyte-derived dendritic cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mLRecombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with 20 ng/mL LPS, 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA), and 20 ng/ml Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hourswere stained with Mouse Anti-Human CD83 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1774F) and either (A) Mouse Anti-Human DC-STAMP APC-conjugated Monoclonal Antibody (Catalog # FAB7824A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human DC-STAMP APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human monocyte-derived dendritic cells treated with Recombinant Human IL-4 (Catalog # 204-IL) and Recombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with LPS, Recombinant Human TNF-alpha (Catalog # 210-TA), and Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hours

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-STAMP

DC-STAMP, also known as TM7SF4, is an approximately 50 kDa glycoprotein with seven transmembrane segments. It is expressed on the surface of dendritic cells and monocytes as well as on osteoclasts and their progenitors. DC-STAMP binds CTGF/CCN2 and cooperates with TRANCE/RANK L for inducing osteoclast differentiation and fusion into multinucleated cells. It contains a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and associates with FC gamma RIII/CD16. Within aa 314-376, human DC-STAMP shares approximately 75% aa sequence identity with mouse and rat DC-STAMP.

Long Name

Dendritic Cell-specific Transmembrane Protein

Alternate Names

DCSTAMP, FIND, IL-4-induced Protein, TM7SF4

Entrez Gene IDs

81501 (Human); 75766 (Mouse); 299889 (Rat)

Gene Symbol

DCSTAMP

UniProt

Q9H295 (Human)

Additional DC-STAMP Products

Product Documents for Human DC-STAMP APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DC-STAMP APC‑conjugated Antibody

For research use only

Related Research Areas

Osteoblast and Osteoclast Markers

Citations for Human DC-STAMP APC‑conjugated Antibody

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