Human DC-STAMP APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7824A
Detection of DC-STAMP in Human Denditic Cells by Flow Cytometry.
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Citations (1)
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Human DC-STAMP APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC-STAMP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse DC-STAMP is observed.
Source
Monoclonal Mouse IgG2B Clone # 788524
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DC-STAMP
Asp314-Thr376
Accession # Q9H295
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of DC-STAMP in Human Denditic Cells by Flow Cytometry. View Larger

Detection of DC-STAMP in Human Denditic Cells by Flow Cytometry. Mature human monocyte-derived dendritic cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mLRecombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with 20 ng/mL LPS, 20 ng/mL Recombinant Human TNF-alpha (Catalog # 210-TA), and 20 ng/ml Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hourswere stained with Mouse Anti-Human CD83 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1774F) and either (A) Mouse Anti-Human DC-STAMP APC‑conjugated Monoclonal Antibody (Catalog # FAB7824A) or (B) Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-STAMP

DC-STAMP, also known as TM7SF4, is an approximately 50 kDa glycoprotein with seven transmembrane segments. It is expressed on the surface of dendritic cells and monocytes as well as on osteoclasts and their progenitors. DC-STAMP binds CTGF/CCN2 and cooperates with TRANCE/RANK L for inducing osteoclast differentiation and fusion into multinucleated cells. It contains a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and associates with FC gamma RIII/CD16. Within aa 314-376, human DC-STAMP shares approximately 75% aa sequence identity with mouse and rat DC-STAMP.

Long Name
Dendritic Cell-specific Transmembrane Protein
Entrez Gene IDs
81501 (Human); 75766 (Mouse); 299889 (Rat)
Alternate Names
DCSTAMP; DC-STAMP; dendritic cell-specific transmembrane protein; FIND; IL-4-induced Protein; TM7SF4; transmembrane 7 superfamily member 4

Product Datasheets

Citation for Human DC-STAMP APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. NF-kappaB-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation.
    Authors: de la Rica L, Garcia-Gomez A, Comet N, Rodriguez-Ubreva J, Ciudad L, Vento-Tormo R, Company C, Alvarez-Errico D, Garcia M, Gomez-Vaquero C, Ballestar E
    Genome Biol, 2015;16(0):2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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