IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
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Recombinant Human IL-1 beta/IL-1F2 Protein
R&D Systems | Catalog # 201-LB
GMP Version Available:
201-GMP
GMP
Analyzed by SEC-MALS
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human IL-1 beta/IL-1F2 Protein (201-LB)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Ala117-Ser269
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala117 & Pro118
Predicted Molecular Mass
17 kDa
SDS-PAGE
17 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED50 for this effect is <12 pg/mL.
The ED50 for this effect is <12 pg/mL.
Reviewed Applications
Read 23 reviews rated 4.7 using 201-LB in the following applications:
- Binding assay/Protein-protein interaction (1 Review)
- Cell Proliferation (3 Reviews)
- Enzymatic activity in vitro (1 Review)
- Immunoassay Standard (2 Reviews)
- In vitro bioactivity in cell culture (10 Reviews)
- In vivo study (1 Review)
- Inflammation activator (1 Review)
- Media additive for protein or antibody production (1 Review)
- Stem/Immune cell maintenance or differentiation (2 Reviews)
Scientific Data Images for Recombinant Human IL-1 beta/IL-1F2 Protein
Recombinant Human IL‑1 beta /IL‑1F2 Protein SEC-MALS.
Recombinant Human IL-1beta Protein (Catalog # 201-LB) has a molecular weight (MW) of 17.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Human IL-1 beta/IL-1F2 Protein Bioactivity
Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.
Recombinant Human IL-1 beta/IL-1F2 Protein SDS-PAGE
1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 201-LB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 201-LB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 50-200 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage |
Calculators
Background: IL-1 beta/IL-1F2
References
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- March, C.J. et al. (1985) Nature 315:641.
- Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Interleukin 1 beta
Alternate Names
IL-1b, IL-1F2, IL1 beta, IL1B
Entrez Gene IDs
Gene Symbol
IL1B
UniProt
Additional IL-1 beta/IL-1F2 Products
Product Documents for Recombinant Human IL-1 beta/IL-1F2 Protein
Product Specific Notices for Recombinant Human IL-1 beta/IL-1F2 Protein
This product is covered by U.S. patent: 5,681,933.
For research use only
Related Research Areas
Citations for Recombinant Human IL-1 beta/IL-1F2 Protein
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Customer Reviews for Recombinant Human IL-1 beta/IL-1F2 Protein (23)
4.7 out of 5
23 Customer Ratings
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Application: Cell ProliferationVerified Customer | Posted 09/12/2024
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 08/26/2024
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Application: In vivo studyVerified Customer | Posted 02/24/2023Human rhIL-1b was used to induce a pro-inflammatory response in mice treated with the cytokine for 4 days (C57BL/6J) in a model of arthritis in vivo. A strong response was shown in histological pictures from the mice pannus.
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Application: Immunoassay StandardVerified Customer | Posted 09/16/2022
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 05/21/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 11/09/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 08/17/2020PBMC assay
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Application: Cell ProliferationVerified Customer | Posted 05/23/2020
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Application: Cell ProliferationVerified Customer | Posted 05/23/2020
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Application: Immunoassay StandardVerified Customer | Posted 01/10/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/07/2020
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Application: Enzymatic activity in vitroVerified Customer | Posted 03/22/2019We used recombinant human IL-1 beta with different concentration (0.5 to 5 ng/ml) to stimulate mouse aorta ring (C57/B6) for 24 hrs, then ROS activity was measured with DHE. It showed dose dependent activation of ROS in smooth muscle cells.
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 11/07/2018
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Application: Media additive for protein or antibody productionVerified Customer | Posted 11/05/2018
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 11/05/2018
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 11/05/2018
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 08/06/2018
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 03/02/2018
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Application: Inflammation activatorVerified Customer | Posted 12/15/2017
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 06/07/2017qPCR
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Verified Customer | Posted 04/24/2017Human Serum used in a dilution of 1/5.
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 12/15/2016Recombination Human IL-1 beta was used to insult iPSc derived Astrocyte to induce cytokine secretion.
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 07/29/2016HUVEC cells were treated with a variety of pro-inflammatory stimuli. IL-1B treatment (right-hand column of each graph) resulted in increased IL-6 and ICAM1 expression, decreased eNOS expression, and massive self-upregulation.
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FAQs for Recombinant Human IL-1 beta/IL-1F2 Protein
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Q: Can I reconstitute Recombinant Human IL-1 beta/IL-1F2 Protein (catalog # 201-LB) at a different concentration than recommended?
A: We cannot guarantee the performance if the protein is reconstituted at a different concentration than recommended in the product datasheet. Please contact Technical Service if you have any questions or concerns about reconstitution. -
A: Yes it does, based on the literature.
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Q: Does Recombinant Human IL-1 beta/IL-1F2 Protein (catalog 201-LB) contain the propeptide?
A: The sequence of this protein is Ala117-Ser269 from Accession # NP_000567. This is the mature form and does not include the propeptide.
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Associated Pathways
IL-1 Family Signaling Pathways
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Inflammasome Activation Pathways
Innate Lymphoid Cell Differentiation Pathways
NOD-like Receptor Signaling Pathways
Th17 Differentiation Pathway
Toll-Like Receptor Signaling Pathways
NOD-like Receptor Signaling Pathways
Th17 Differentiation Pathway
Toll-Like Receptor Signaling Pathways