Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 259931
View all Dectin-1/CLEC7A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A
Thr66-Met201
Accession # NP_072092

Specificity

Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Dectin‑1/CLEC7A APC‑conjugated Antibody

Detection of Dectin-1/CLEC7A antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of Dectin‑1/CLEC7A in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human Dectin-1/CLEC7A APC-conjugated Monoclonal Antibody (Catalog # FAB1859A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Dectin‑1/CLEC7A APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes

Reviewed Applications

Read 1 review rated 5 using FAB1859A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

References

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Alternate Names

CD369, CLEC7A, CLECSF12, Dectin1

Entrez Gene IDs

64581 (Human); 56644 (Mouse)

Gene Symbol

CLEC7A

UniProt

NP_072092

Additional Dectin-1/CLEC7A Products

Product Documents for Human Dectin‑1/CLEC7A APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Dectin‑1/CLEC7A APC‑conjugated Antibody

For research use only

Citations for Human Dectin‑1/CLEC7A APC‑conjugated Antibody

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  • Human Dectin-1/CLEC7A APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Verified Customer | Posted 01/16/2024
    Detection of human Dectin1 on HEK cells overexpressing isoform A of human Dectin1. The cells were treated with 5 μl of the human Dectin1 APC-conjugated antibody (catalogue # FAB1859A) (BLUE) or mouse IgG2b APC-conjugated isotype control antibody (RED)
    Human Dectin‑1/CLEC7A APC‑conjugated Antibody FAB1859A

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