Human Dectin-1/CLEC7A PE-conjugated Antibody

Clone 259931 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB1859P-100
FAB1859P-025
Detection of Dectin‑1/CLEC7A in Human Blood Monocytes by Flow Cytometry.
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Citations (8)
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Human Dectin-1/CLEC7A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 259931
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A
Thr66-Met201
Accession # NP_072092
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Dectin‑1/CLEC7A in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of Dectin‑1/CLEC7A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Dectin‑1/CLEC7A PE-conjugated Monoclonal Antibody (Catalog # FAB1859P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

Product Datasheets

Citations for Human Dectin-1/CLEC7A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Isoform localization of Dectin-1 regulates the signaling quality of anti-fungal immunity
    Authors: M Fischer, JP Müller, B Spies-Weis, C Gräfe, O Kurzai, K Hünniger, A Hochhaus, S Scholl, U Schnetzke
    Eur. J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
    PLoS ONE, 2015;10(3):e0119244.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  3. Opposite regulatory effects of IFN-beta and IL-3 on C-type lectin receptors, antigen uptake, and phagocytosis in human macrophages.
    Authors: Cardone M, Ikeda K, Varano B, Belardelli F, Millefiorini E, Gessani S, Conti L
    J Leukoc Biol, 2014;95(1):161-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. Ex vivo cytokine release and pattern recognition receptor expression of subjects exposed to dampness: pilot study to assess the outcome of mould exposure to the innate immune system.
    Authors: Punsmann S, Liebers V, Lotz A, Bruning T, Raulf M
    PLoS ONE, 2013;8(12):e82734.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  5. MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
    Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
    PLoS ONE, 2012;7(12):e50589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  6. Vitamin D(3) down-regulates proinflammatory cytokine response to Mycobacterium tuberculosis through pattern recognition receptors while inducing protective cathelicidin production.
    Authors: Khoo AL, Chai LY, Koenen HJ, Oosting M, Steinmeyer A, Zuegel U, Joosten I, Netea MG, van der Ven AJ
    Cytokine, 2011;55(2):294-300.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  7. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
    Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
    Immunol. Lett., 2011;134(2):113-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  8. Dectin-1 Y238X polymorphism associates with susceptibility to invasive aspergillosis in hematopoietic transplantation through impairment of both recipient- and donor-dependent mechanisms of antifungal immunity.
    Authors: Cunha C, Di Ianni M, Bozza S, Giovannini G, Zagarella S, Zelante T, D'Angelo C, Pierini A, Pitzurra L, Falzetti F, Carotti A, Perruccio K, Latge JP, Rodrigues F, Velardi A, Aversa F, Romani L, Carvalho A
    Blood, 2010;116(24):5394-402.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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