Human Dectin-1/CLEC7A Antibody (259931)

R&D Systems | Catalog # MAB1859

Clone 259931 was used by HLDA to establish CD designation
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Blockade of Receptor-ligand Interaction, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Bioassay, Blocking, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 259931
View all Dectin-1/CLEC7A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A
Thr66-Met201
Accession # NP_072092

Specificity

Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Dectin-1/CLEC7A Antibody (259931)

Dectin-1/CLEC7A antibody in Human Dendritic Cells by Immunocytochemistry (ICC).

Dectin‑1/CLEC7A in Human Dendritic Cells.

Dectin-1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of hcxtnxqqrfkwbdd2uwhc.jpg by Flow Cytometry

Detection of hcxtnxqqrfkwbdd2uwhc.jpg by Flow Cytometry

Glycosylation of the stalk region of hDectin-1 mediates recognition by CLEC-2.(A) Schematic representations of mouse Dectin-1 (mD1), human Dectin-1 (hD1), and two chimeric mutants (m-hD1, and h-mD1) are shown in the left panel. NFAT-GFP reporter cells expressing these constructs were incubated with zymosan (right panel). ‘–’: cells without stimulants. (B) Schematic representations of hD1A and hD1B are shown in the left panel. NFAT-GFP reporter cells expressing hD1 were incubated with zymosan (right panel). ‘–’: cells without stimulants. (C) Schematic representations of hD1A delta CRD and hD1A are shown in the left panel. NFAT-GFP reporter cells expressing these constructs were incubated with zymosan (right panel). ‘–’: cells without stimulants. (D) The surface expression levels of hDectin-1 in wild-type (WT), COSMC KO (COSMC−/−), recaptured COSMC (COSMC−/− + COSMC), and C2GnT overexpressed cells were evaluated using anti-hDectin-1 antibody (gray histograms). Open histograms represent unstained controls. (E) The surface expression levels of hDectin-1 in non-treated (open histogram) and sialidase-treated cells (gray histogram) were evaluated using an anti-hDectin-1 antibody (left panel). Non-treated or sialidase-treated NFAT-GFP reporter cells expressing hDectin-1 were incubated with zymosan (right panel). ‘–’: cells without indicated stimulants. Data are presented as mean ± SD (A, B, C, and E). These results are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36479973), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Dectin-1/CLEC7A by Flow Cytometry

Detection of Dectin-1/CLEC7A by Flow Cytometry

Glycosylation of the stalk region of hDectin-1 mediates recognition by CLEC-2.(A) Schematic representations of mouse Dectin-1 (mD1), human Dectin-1 (hD1), and two chimeric mutants (m-hD1, and h-mD1) are shown in the left panel. NFAT-GFP reporter cells expressing these constructs were incubated with zymosan (right panel). ‘–’: cells without stimulants. (B) Schematic representations of hD1A and hD1B are shown in the left panel. NFAT-GFP reporter cells expressing hD1 were incubated with zymosan (right panel). ‘–’: cells without stimulants. (C) Schematic representations of hD1A delta CRD and hD1A are shown in the left panel. NFAT-GFP reporter cells expressing these constructs were incubated with zymosan (right panel). ‘–’: cells without stimulants. (D) The surface expression levels of hDectin-1 in wild-type (WT), COSMC KO (COSMC−/−), recaptured COSMC (COSMC−/− + COSMC), and C2GnT overexpressed cells were evaluated using anti-hDectin-1 antibody (gray histograms). Open histograms represent unstained controls. (E) The surface expression levels of hDectin-1 in non-treated (open histogram) and sialidase-treated cells (gray histogram) were evaluated using an anti-hDectin-1 antibody (left panel). Non-treated or sialidase-treated NFAT-GFP reporter cells expressing hDectin-1 were incubated with zymosan (right panel). ‘–’: cells without indicated stimulants. Data are presented as mean ± SD (A, B, C, and E). These results are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36479973), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Dectin-1/CLEC7A by Flow Cytometry

Detection of Dectin-1/CLEC7A by Flow Cytometry

Glycosylation of the stalk region of hDectin-1 mediates recognition by CLEC-2.(A) Schematic representations of mouse Dectin-1 (mD1), human Dectin-1 (hD1), and two chimeric mutants (m-hD1, and h-mD1) are shown in the left panel. NFAT-GFP reporter cells expressing these constructs were incubated with zymosan (right panel). ‘–’: cells without stimulants. (B) Schematic representations of hD1A and hD1B are shown in the left panel. NFAT-GFP reporter cells expressing hD1 were incubated with zymosan (right panel). ‘–’: cells without stimulants. (C) Schematic representations of hD1A delta CRD and hD1A are shown in the left panel. NFAT-GFP reporter cells expressing these constructs were incubated with zymosan (right panel). ‘–’: cells without stimulants. (D) The surface expression levels of hDectin-1 in wild-type (WT), COSMC KO (COSMC−/−), recaptured COSMC (COSMC−/− + COSMC), and C2GnT overexpressed cells were evaluated using anti-hDectin-1 antibody (gray histograms). Open histograms represent unstained controls. (E) The surface expression levels of hDectin-1 in non-treated (open histogram) and sialidase-treated cells (gray histogram) were evaluated using an anti-hDectin-1 antibody (left panel). Non-treated or sialidase-treated NFAT-GFP reporter cells expressing hDectin-1 were incubated with zymosan (right panel). ‘–’: cells without indicated stimulants. Data are presented as mean ± SD (A, B, C, and E). These results are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36479973), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Dectin-1/CLEC7A Antibody (259931)

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood monocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and mature human dendritic cells

Reviewed Applications

Read 5 reviews rated 4.8 using MAB1859 in the following applications:

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

References

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Alternate Names

CD369, CLEC7A, CLECSF12, Dectin1

Entrez Gene IDs

64581 (Human); 56644 (Mouse)

Gene Symbol

CLEC7A

UniProt

NP_072092

Additional Dectin-1/CLEC7A Products

Product Documents for Human Dectin-1/CLEC7A Antibody (259931)

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Product Specific Notices for Human Dectin-1/CLEC7A Antibody (259931)

For research use only

Citations for Human Dectin-1/CLEC7A Antibody (259931)

Customer Reviews for Human Dectin-1/CLEC7A Antibody (259931) (5)

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Showing  1 - 5 of 5 reviews Showing All
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  • Human Dectin-1/CLEC7A Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Verified Customer | Posted 01/16/2024
    Detection of human Dectin1 on HEK cells overexpressing isoform A of human Dectin1. The cells were treated with 100 nM of the human Dectin1 (catalogue # MAB1859) (BLUE) or mouse IgG2b isotype control antibody (RED) followed by treatments with a secondary goat anti-mouse IgG AF647-conjugated antibody
    Human Dectin-1/CLEC7A Antibody (259931) MAB1859
  • Human Dectin-1/CLEC7A Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 11/28/2020
    Dectin1 is detected in THP1 monocytes using 100 nM of the Human Dectin-1/CLEC7A Antibody clone 259931. The 259931 antibody was detected with a secondary goat anti-mouse IgG Fc Alexa Fluor 647 antibody.
    Human Dectin-1/CLEC7A Antibody (259931) MAB1859
  • Human Dectin-1/CLEC7A Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: macrophages
    Species: Human
    Verified Customer | Posted 10/13/2020
    Detection of human Dectin­1 in monocyte-derived cultured macrophages by Flow Cytometry. Human monocytes were cultured in MCSF for 6 days to differentiate into macrophages. After differentiation, the macrophages were stained with the mouse anti-human Dectin­1 antibody clone 259931 at 100 nM or the mouse IgG2b isotype control. Binding was detected with a secondary goat a-mouse IgG Fc gamma fragment APC antibody.
    Human Dectin-1/CLEC7A Antibody (259931) MAB1859
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23514738
    Species: Other
    Verified Customer | Posted 02/12/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 24424029
    Species: Human
    Verified Customer | Posted 02/12/2015

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