Human Dectin-1/CLEC7A Antibody

Clone 259931 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1859
MAB1859-SP
Human Dectin-1/CLEC7A Antibody in Data
1 Image
Product Details
Citations (22)
FAQs
Supplemental Products
Reviews (2)

Human Dectin-1/CLEC7A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 259931
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A
Thr66-Met201
Accession # NP_072092
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Dectin‑1/CLEC7A in Human Dendritic Cells. View Larger

Dectin‑1/CLEC7A in Human Dendritic Cells. Dectin-1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

Product Datasheets

Citations for Human Dectin-1/CLEC7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Mac-1 triggers neutrophil DNA extracellular trap formation to Aspergillus fumigatus independently of PAD4 histone citrullination
    Authors: JC Silva, NC Rodrigues, GA Thompson-S, VS Muniz, JS Neves, RT Figueiredo
    J. Leukoc. Biol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis
    Authors: JC de Quaglia, AM Della Cole, TP Gardizani, GG Romagnoli, R Kaneno, LA Dias-Melic
    J Immunol Res, 2019;2019(0):1529189.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Cellular metabolism constrains innate immune responses in early human ontogeny
    Authors: B Kan, C Michalski, H Fu, HHT Au, K Lee, EA Marchant, MF Cheng, E Anderson-B, M Aharoni-Si, P Tilley, RG Mirmira, CJ Ross, DS Luciani, E Jan, PM Lavoie
    Nat Commun, 2018;9(1):4822.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses
    Authors: MMM Kaisar, M Ritter, C Del Fresno, HS Jónasdótti, AJ van der Ha, LR Pelgrom, G Schramm, LE Layland, D Sancho, C Prazeres d, M Giera, M Yazdanbakh, B Everts
    PLoS Biol., 2018;16(4):e2005504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells
    Authors: CC Yeh, HC Horng, H Chou, HY Tai, HD Shen, SL Hsieh, PH Wang
    Int J Mol Sci, 2017;18(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. EphA2 is an epithelial cell pattern recognition receptor for fungal ?-glucans
    Authors: M Swidergall, NV Solis, MS Lionakis, SG Filler
    Nat Microbiol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.
    Authors: Gazendam R, van Hamme J, Tool A, Hoogenboezem M, van den Berg J, Prins J, Vitkov L, van de Veerdonk F, van den Berg T, Roos D, Kuijpers T
    J Immunol, 2016;196(3):1272-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
    PLoS ONE, 2016;11(11):e0167410.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.
    Authors: Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li X, Hori T, Zurawski S, Liu Y, Zurawski G, Oh S
    J Immunol, 2015;195(4):1723-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response.
    Authors: Elder M, Webster S, Williams D, Gaston J, Goodall J
    Eur J Immunol, 2015;46(2):455-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
    Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
    PLoS ONE, 2015;10(3):e0120948.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  12. Podosomes of dendritic cells facilitate antigen sampling.
    Authors: Baranov, Maksim V, Ter Beest, Martin, Reinieren-Beeren, Inge, Cambi, Alessand, Figdor, Carl G, van den Bogaart, Geert
    J Cell Sci, 2014;127(0):1052-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis.
    Authors: Rodrigues D, Fernandes R, Balderramas H, Penitenti M, Bachiega T, Calvi S, Dias-Melicio L, Ikoma M, Soares A
    Cytokine, 2014;69(1):102-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Human intestinal epithelial cells respond to beta-glucans via Dectin-1 and Syk.
    Authors: Cohen-Kedar S, Baram L, Elad H, Brazowski E, Guzner-Gur H, Dotan I
    Eur J Immunol, 2014;44(12):3729-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum.
    Authors: Lefevre L, Lugo-Villarino G, Meunier E, Valentin A, Olagnier D, Authier H, Duval C, Dardenne C, Bernad J, Lemesre J, Auwerx J, Neyrolles O, Pipy B, Coste A
    Immunity, 2013;38(5):1038-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway.
    Authors: Ifrim D, Joosten L, Kullberg B, Jacobs L, Jansen T, Williams D, Gow N, van der Meer J, Netea M, Quintin J
    J Immunol, 2013;190(8):4129-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1beta via a noncanonical caspase-8 inflammasome.
    Authors: Gringhuis SI, Kaptein TM, Wevers BA, Theelen B, van der Vlist M, Boekhout T, Geijtenbeek TB
    Nat. Immunol., 2012;13(3):246-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli.
    Authors: Kassianos AJ, Hardy MY, Ju X, Vijayan D, Ding Y, Vulink AJ, McDonald KJ, Jongbloed SL, Wadley RB, Wells C, Hart DN, Radford KJ
    Eur. J. Immunol., 2012;42(6):1512-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
    Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
    PLoS ONE, 2012;7(12):e50589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2.
    Authors: Gringhuis SI, Wevers BA, Kaptein TM, van Capel TM, Theelen B, Boekhout T, de Jong EC, Geijtenbeek TB
    PLoS Pathog., 2011;7(1):e1001259.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes.
    Authors: Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK
    J. Leukoc. Biol., 2011;90(3):599-611.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  22. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2011;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Dectin-1/CLEC7A Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Dectin-1/CLEC7A Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Dectin-1/CLEC7A Antibody
By Anonymous on 11/28/2020
Application: Flow Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

Dectin1 is detected in THP1 monocytes using 100 nM of the Human Dectin-1/CLEC7A Antibody clone 259931. The 259931 antibody was detected with a secondary goat anti-mouse IgG Fc Alexa Fluor 647 antibody.


Human Dectin-1/CLEC7A Antibody
By Anonymous on 10/13/2020
Application: Flow Sample Tested: macrophages Species: Human

Detection of human Dectin­1 in monocyte-derived cultured macrophages by Flow Cytometry. Human monocytes were cultured in MCSF for 6 days to differentiate into macrophages. After differentiation, the macrophages were stained with the mouse anti-human Dectin­1 antibody clone 259931 at 100 nM or the mouse IgG2b isotype control. Binding was detected with a secondary goat a-mouse IgG Fcγ fragment APC antibody.