Human Dectin-1/CLEC7A Antibody
Human Dectin-1/CLEC7A Antibody Summary
Thr66-Met201
Accession # NP_072092
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Dectin‑1/CLEC7A in Human Dendritic Cells. Dectin-1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dectin-1/CLEC7A
Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.
- Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
- Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
- Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
- Yokota, K. et al. (2001) Gene 272:51.
- Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
- Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
- Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
- Gantner, B.N. et al. (2005) EMBO J. 24:1277.
- Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
- Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
- Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
- Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
- Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
- Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
- Saijo, S. et al. (2007) Nat. Immunol. 8:39.
- Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Product Datasheets
Citations for Human Dectin-1/CLEC7A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
Nature Immunology, 2022;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human Dectin-1 is O-glycosylated and serves as a ligand for C-type lectin receptor CLEC-2
Authors: S Haji, T Ito, C Guenther, M Nakano, T Shimizu, D Mori, Y Chiba, M Tanaka, SK Mishra, JA Willment, GD Brown, M Nagae, S Yamasaki
Elife, 2022;11(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
LYSMD3: A mammalian pattern recognition receptor for chitin
Authors: X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence
Cell Reports, 2021;36(3):109392.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Essential role of TOSO/FAIM3 in intestinal IgM reverse transcytosis
Authors: N Rochereau, E Michaud, L Waeckel, M Killian, R Gayet, R Goguyer-De, X Roblin, G Biolley, B Corthésy, S Paul
Cell Reports, 2021;37(7):110006.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Mac-1 triggers neutrophil DNA extracellular trap formation to Aspergillus fumigatus independently of PAD4 histone citrullination
Authors: JC Silva, NC Rodrigues, GA Thompson-S, VS Muniz, JS Neves, RT Figueiredo
J. Leukoc. Biol., 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis
Authors: JC de Quaglia, AM Della Cole, TP Gardizani, GG Romagnoli, R Kaneno, LA Dias-Melic
J Immunol Res, 2019;2019(0):1529189.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses
Authors: MMM Kaisar, M Ritter, C Del Fresno, HS Jónasdótti, AJ van der Ha, LR Pelgrom, G Schramm, LE Layland, D Sancho, C Prazeres d, M Giera, M Yazdanbakh, B Everts
PLoS Biol., 2018;16(4):e2005504.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Cellular metabolism constrains innate immune responses in early human ontogeny
Authors: B Kan, C Michalski, H Fu, HHT Au, K Lee, EA Marchant, MF Cheng, E Anderson-B, M Aharoni-Si, P Tilley, RG Mirmira, CJ Ross, DS Luciani, E Jan, PM Lavoie
Nat Commun, 2018;9(1):4822.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells
Authors: CC Yeh, HC Horng, H Chou, HY Tai, HD Shen, SL Hsieh, PH Wang
Int J Mol Sci, 2017;18(3):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
EphA2 is an epithelial cell pattern recognition receptor for fungal ?-glucans
Authors: M Swidergall, NV Solis, MS Lionakis, SG Filler
Nat Microbiol, 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.
Authors: Gazendam R, van Hamme J, Tool A, Hoogenboezem M, van den Berg J, Prins J, Vitkov L, van de Veerdonk F, van den Berg T, Roos D, Kuijpers T
J Immunol, 2016;196(3):1272-83.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
PLoS ONE, 2016;11(11):e0167410.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.
Authors: Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li X, Hori T, Zurawski S, Liu Y, Zurawski G, Oh S
J Immunol, 2015;195(4):1723-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
PLoS ONE, 2015;10(3):e0120948.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response.
Authors: Elder M, Webster S, Williams D, Gaston J, Goodall J
Eur J Immunol, 2015;46(2):455-63.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis.
Authors: Rodrigues D, Fernandes R, Balderramas H, Penitenti M, Bachiega T, Calvi S, Dias-Melicio L, Ikoma M, Soares A
Cytokine, 2014;69(1):102-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human intestinal epithelial cells respond to beta-glucans via Dectin-1 and Syk.
Authors: Cohen-Kedar S, Baram L, Elad H, Brazowski E, Guzner-Gur H, Dotan I
Eur J Immunol, 2014;44(12):3729-40.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Podosomes of dendritic cells facilitate antigen sampling.
Authors: Baranov, Maksim V, Ter Beest, Martin, Reinieren-Beeren, Inge, Cambi, Alessand, Figdor, Carl G, van den Bogaart, Geert
J Cell Sci, 2014;127(0):1052-64.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum.
Authors: Lefevre L, Lugo-Villarino G, Meunier E, Valentin A, Olagnier D, Authier H, Duval C, Dardenne C, Bernad J, Lemesre J, Auwerx J, Neyrolles O, Pipy B, Coste A
Immunity, 2013;38(5):1038-49.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway.
Authors: Ifrim D, Joosten L, Kullberg B, Jacobs L, Jansen T, Williams D, Gow N, van der Meer J, Netea M, Quintin J
J Immunol, 2013;190(8):4129-35.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
PLoS ONE, 2012;7(12):e50589.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1beta via a noncanonical caspase-8 inflammasome.
Authors: Gringhuis SI, Kaptein TM, Wevers BA, Theelen B, van der Vlist M, Boekhout T, Geijtenbeek TB
Nat. Immunol., 2012;13(3):246-54.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli.
Authors: Kassianos AJ, Hardy MY, Ju X, Vijayan D, Ding Y, Vulink AJ, McDonald KJ, Jongbloed SL, Wadley RB, Wells C, Hart DN, Radford KJ
Eur. J. Immunol., 2012;42(6):1512-22.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes.
Authors: Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK
J. Leukoc. Biol., 2011;90(3):599-611.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2.
Authors: Gringhuis SI, Wevers BA, Kaptein TM, van Capel TM, Theelen B, Boekhout T, de Jong EC, Geijtenbeek TB
PLoS Pathog., 2011;7(1):e1001259.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
Immunol. Lett., 2011;134(2):167-73.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Reviews for Human Dectin-1/CLEC7A Antibody
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Dectin1 is detected in THP1 monocytes using 100 nM of the Human Dectin-1/CLEC7A Antibody clone 259931. The 259931 antibody was detected with a secondary goat anti-mouse IgG Fc Alexa Fluor 647 antibody.
Detection of human Dectin1 in monocyte-derived cultured macrophages by Flow Cytometry. Human monocytes were cultured in MCSF for 6 days to differentiate into macrophages. After differentiation, the macrophages were stained with the mouse anti-human Dectin1 antibody clone 259931 at 100 nM or the mouse IgG2b isotype control. Binding was detected with a secondary goat a-mouse IgG Fcγ fragment APC antibody.
