Human Dectin-1/CLEC7A Antibody

Clone 259931 was used by HLDA to establish CD designation.
(17 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 259931
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A
    Thr66-Met201
    Accession # NP_072092
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood monocytes
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Dectin‑1/CLEC7A in Human Dendritic Cells. Dectin‑1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin‑1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

  • References:
    1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
    2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
    3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
    4. Yokota, K. et al. (2001) Gene 272:51.
    5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
    6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
    7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
    8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
    9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
    10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
    11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
    12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
    13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
    14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
    15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
    16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
  • Entrez Gene IDs:
    64581 (Human); 56644 (Mouse)
  • Alternate Names:
    Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells
    Authors: CC Yeh, HC Horng, H Chou, HY Tai, HD Shen, SL Hsieh, PH Wang
    Int J Mol Sci, 2017;18(3):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
    PLoS ONE, 2016;11(11):e0167410.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.
    Authors: Gazendam R, van Hamme J, Tool A, Hoogenboezem M, van den Berg J, Prins J, Vitkov L, van de Veerdonk F, van den Berg T, Roos D, Kuijpers T
    J Immunol, 2016;196(3):1272-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.
    Authors: Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li X, Hori T, Zurawski S, Liu Y, Zurawski G, Oh S
    J Immunol, 2015;195(4):1723-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response.
    Authors: Elder M, Webster S, Williams D, Gaston J, Goodall J
    Eur J Immunol, 2015;46(2):455-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
    Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
    PLoS ONE, 2015;10(3):e0120948.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  7. Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis.
    Authors: Rodrigues D, Fernandes R, Balderramas H, Penitenti M, Bachiega T, Calvi S, Dias-Melicio L, Ikoma M, Soares A
    Cytokine, 2014;69(1):102-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Podosomes of dendritic cells facilitate antigen sampling.
    Authors: Baranov, Maksim V, Ter Beest, Martin, Reinieren-Beeren, Inge, Cambi, Alessand, Figdor, Carl G, van den Bogaart, Geert
    J Cell Sci, 2014;127(0):1052-64.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Human intestinal epithelial cells respond to beta-glucans via Dectin-1 and Syk.
    Authors: Cohen-Kedar S, Baram L, Elad H, Brazowski E, Guzner-Gur H, Dotan I
    Eur J Immunol, 2014;44(12):3729-40.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  10. Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway.
    Authors: Ifrim D, Joosten L, Kullberg B, Jacobs L, Jansen T, Williams D, Gow N, van der Meer J, Netea M, Quintin J
    J Immunol, 2013;190(8):4129-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum.
    Authors: Lefevre L, Lugo-Villarino G, Meunier E, Valentin A, Olagnier D, Authier H, Duval C, Dardenne C, Bernad J, Lemesre J, Auwerx J, Neyrolles O, Pipy B, Coste A
    Immunity, 2013;38(5):1038-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1beta via a noncanonical caspase-8 inflammasome.
    Authors: Gringhuis SI, Kaptein TM, Wevers BA, Theelen B, van der Vlist M, Boekhout T, Geijtenbeek TB
    Nat. Immunol., 2012;13(3):246-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli.
    Authors: Kassianos AJ, Hardy MY, Ju X, Vijayan D, Ding Y, Vulink AJ, McDonald KJ, Jongbloed SL, Wadley RB, Wells C, Hart DN, Radford KJ
    Eur. J. Immunol., 2012;42(6):1512-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
    Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
    PLoS ONE, 2012;7(12):e50589.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  15. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2011;134(2):167-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2.
    Authors: Gringhuis SI, Wevers BA, Kaptein TM, van Capel TM, Theelen B, Boekhout T, de Jong EC, Geijtenbeek TB
    PLoS Pathog., 2011;7(1):e1001259.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  17. Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes.
    Authors: Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK
    J. Leukoc. Biol., 2011;90(3):599-611.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
Expand to show all 17 Citations
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