Human DGK-alpha Antibody

Name: Human DGK-alpha Antibody
Brand: R&D Systems
SKU: MAB6597

Catalog #: MAB6597 Datasheet / COA / SDS

Discontinued Product

MAB6597 has been discontinued.

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Detection of Human DGK-alpha by Western Blot.

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Human DGK-alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human DGK-alpha in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 612413

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DGK-alpha
Met1-Glu162 (predicted)
Accession # NP_001336

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DGK-alpha by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and BJAB human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Human DGK-a Monoclonal Antibody (Catalog # MAB6597) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-a at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGK-alpha

DGK-alpha (Diacylglycerol kinase alpha) is an 80 kDa, 735 amino acid (aa), type I member of the eukaryocytic diacylglycerol kinase family of enzymes, possessing EF‑hand, C1/Cys-rich zinc finger and catalytic domains. In T cells, IL-2 stimulates its translocation to the nucleus, promoting proliferation. TCR stimulation, however, promotes migration to the plasma membrane to downregulate RAS activation and maintain anergy. It also participates in VEGF or HGF-mediated motility in endothelial, epithelial, or cancer cells. Over aa 1-162, which includes the first of two EF‑hand motifs, human DGK-a shares ~75% aa identity with mouse and rat DGK‑ alpha.

Long Name

Diacylglycerol Kinase, alpha 80kDa

Entrez Gene IDs

1606 (Human)

Alternate Names

DAG kinase alpha; DAGK; DAGK1; DAGK1diacylglycerol kinase, alpha (80kD); DGKA; DGKalpha; DGK-alpha; DGK-alphaEC 2.7.1.107; diacylglycerol kinase alpha; diacylglycerol kinase, alpha 80kDa; Diglyceride kinase alpha; MGC12821,80 kDa diacylglycerol kinase; MGC42356

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

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