Human DGK-alpha Antibody

MAB6597 has been discontinued. View all DGK-alpha products.
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human DGK-alpha in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 612413
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human DGK-alpha
    Met1-Glu162 (predicted)
    Accession # NP_001336
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human DGK-alpha by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and BJAB human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Human DGK-alpha Monoclonal Antibody (Catalog # MAB6597) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-alpha at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DGK-alpha
DGK-alpha (Diacylglycerol kinase alpha) is an 80 kDa, 735 amino acid (aa), type I member of the eukaryocytic diacylglycerol kinase family of enzymes, possessing EF‑hand, C1/Cys-rich zinc finger and catalytic domains. In T cells, IL-2 stimulates its translocation to the nucleus, promoting proliferation. TCR stimulation, however, promotes migration to the plasma membrane to downregulate RAS activation and maintain anergy.  It also participates in VEGF or HGF-mediated motility in endothelial, epithelial, or cancer cells. Over aa 1-162, which includes the first of two EF‑hand motifs, human DGK-a shares ~75% aa identity with mouse and rat DGK‑ alpha.
  • Long Name:
    Diacylglycerol Kinase, alpha 80kDa
  • Entrez Gene IDs:
    1606 (Human)
  • Alternate Names:
    DAG kinase alpha; DAGK; DAGK1; DAGK1diacylglycerol kinase, alpha (80kD); DGKA; DGKalpha; DGK-alpha; DGK-alphaEC 2.7.1.107; diacylglycerol kinase alpha; diacylglycerol kinase, alpha 80kDa; Diglyceride kinase alpha; MGC12821,80 kDa diacylglycerol kinase; MGC42356
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