Human DOK4 Antibody Summary
Asp190-Gln326
Accession # Q8TEW6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human DOK4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DOK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4826) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DOK4 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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DOK4 in Human Kidney. DOK4 was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Sheep Anti-Human DOK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4826) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background:
DOK4 (Downstream of kinase 4; also IRS5) is a 41 kDa member of the type B subfamily, DOK family of proteins. It is expressed in T cells and endothelium, plus embryonic neurons and renal anlage, and serves as an intermediary between the InsR and downstream MAP kinase activation. Human DOK4 is 326 amino acids (aa) in length. It contains an N-terminal insulin receptor substrate (IRS) pleckstrin homology domain (aa 7-109) and a centrally placed IRS phosphotyrosine-binding domain (aa 125-236). There is a potential alternate start site at Met16. Over aa 190-326, human DOK4 shares 96% and 98% aa sequence identity with mouse and canine DOK4, respectively.
Product Datasheets
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