Human DPPA4 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human DPPA4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse DPPA4 is observed and less than 1% cross-reactivity with recombinant human DPPA5 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human DPPA4
    Met1-Glu304
    Accession # Q7L190
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human DPPA4
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of DPPA4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Sheep Anti-Human DPPA4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3674, filled histogram) or isotype control antibody ???, followed by Streptavidin-Phycoerythrin (Catalog # F0040). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPPA4
DPPA molecules are a group of five name-only related proteins that are best described as a set of Oct4-related genes. They serve as markers for early embryonic and germline pluripotent cells. DPPA1 is transmembrane, DPPA2-4 contain DNA-binding domains, while DPPA5/ESG1 is a K homology RNA-binding (KH) domain containing protein that is expressed in embryonic germ cells and embryonic stem cells. DPPA5 is a potential marker for pluripotent stem cells.
  • Long Name:
    Developmental Pluripotency Associated 4
  • Entrez Gene IDs:
    55211 (Human); 73693 (Mouse)
  • Alternate Names:
    developmental pluripotency associated 4; developmental pluripotency-associated protein 4; DPPA4; FLJ10713,2410091M23Rik
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Biotinylated Control

Ctrl BAF020 1
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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