DPPA4 is a nuclear SAP-domain protein whose expression is restricted to pluripotent cells, undifferentiated embryonal carcinomas and germ cell tumors. It is considered a marker for embryonic stem cells. Human DPPA4 shares 51% amino acid sequence homology with mouse DPPA4.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Intracellular Staining by Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human DPPA4
Met1-Glu304
Accession # Q7L190
Met1-Glu304
Accession # Q7L190
Specificity
Detects human DPPA4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse DPPA4 is observed and less than 1% cross-reactivity with recombinant human DPPA5 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human DPPA4 Biotinylated Antibody
Detection of DPPA4 in NTera‑2 Human Cell Line by Flow Cytometry.
NTera-2 human testicular embryonic carcinoma cell line was stained with Sheep Anti-Human DPPA4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3674, filled histogram) or isotype control antibody (Catalog # BAF020), followed by Streptavidin-Phycoerythrin (Catalog # F0040). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Applications for Human DPPA4 Biotinylated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: NTera‑2 human testicular embryonic carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.1 µg/mL
Sample: Recombinant Human DPPA4
Sample: Recombinant Human DPPA4
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DPPA4
Long Name
Developmental Pluripotency Associated 4
Alternate Names
developmental pluripotency associated 4, developmental pluripotency-associated protein 4, FLJ10713,2410091M23Rik
Gene Symbol
DPPA4
UniProt
Additional DPPA4 Products
Product Documents for Human DPPA4 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human DPPA4 Biotinylated Antibody
For research use only
Related Research Areas
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