Detects human DPPA4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse DPPA4 is observed and less than 1% cross-reactivity with recombinant human DPPA5 is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant human DPPA4 Met1-Glu304 Accession # Q7L190
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human DPPA4
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of DPPA4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Sheep Anti-Human DPPA4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3674, filled histogram) or isotype control antibody ???, followed by Streptavidin-Phycoerythrin (Catalog # F0040). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DPPA molecules are a group of five name-only related proteins that are best described as a set of Oct4-related genes. They serve as markers for early embryonic and germline pluripotent cells. DPPA1 is transmembrane, DPPA2-4 contain DNA-binding domains, while DPPA5/ESG1 is a K homology RNA-binding (KH) domain containing protein that is expressed in embryonic germ cells and embryonic stem cells. DPPA5 is a potential marker for pluripotent stem cells.
Developmental Pluripotency Associated 4
Entrez Gene IDs:
55211 (Human); 73693 (Mouse)
developmental pluripotency associated 4; developmental pluripotency-associated protein 4; DPPA4; FLJ10713,2410091M23Rik
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.