Human DPPA4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human DPPA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse DPPA4 is observed and less than 1% cross-reactivity with recombinant human DPPA5 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human DPPA4
    Met1-Glu304
    Accession # Q7L190
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human DPPA4
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of DPPA4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Sheep Anti-Human DPPA4 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3674, filled histogram) or isotype control antibody (Catalog #
5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Immunocytochemistry
DPPA4 in Human iPSK3 cells. DPPA4 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Sheep Anti-Human DPPA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3674) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPPA4
DPPA4 is a nuclear SAP-domain protein whose expression is restricted to pluripotent cells, undifferentiated embryonal carcinomas and germ cell tumors. It is considered a marker for embryonic stem cells. Human DPPA4 shares 51% amino acid sequence homology with mouse DPPA4.
  • Long Name:
    Developmental Pluripotency Associated 4
  • Entrez Gene IDs:
    55211 (Human); 73693 (Mouse)
  • Alternate Names:
    developmental pluripotency associated 4; developmental pluripotency-associated protein 4; DPPA4; FLJ10713,2410091M23Rik
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