Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.
Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)
Protein A or G purified from hybridoma culture supernatant
Activated HUVEC human umbilical vein endothelial cells
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of E‑Selectin/P‑Selectin (CD62E/P) in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA) were stained with Mouse Anti-Human E‑Selectin/P‑Selectin (CD62E/P) APC‑conjugated Monoclonal Antibody (Catalog # FAB6169A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: E-Selectin (CD62E)/P-Selectin (CD62P)
E-Selectin (CD62E) and P-Selectin (CD62P) are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C-type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.
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