Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Antibody

  (2 citations)     
Datasheet / CoA / SDS
Product Details
Citations (2)
Supplemental Products
  • Species Reactivity
  • Specificity
    Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.
  • Source
    Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of E‑Selectin/P‑Selectin (CD62E/P) in HUVEC Human Umbilical Vein Endothelial Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells stained with TNF-alpha was stained with Mouse Anti-Human E‑Selectin/P‑Selectin (CD62E/P) Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB6169F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: E-Selectin (CD62E)/P-Selectin (CD62P)
E-Selectin (CD62E) and P-Selectin (CD62P) are members of the Selectin/LECAM family of molecules. E- and P-Selectin are type I transmembrane glycoproteins that contain an N-terminal calcium-dependent C-type lectin domain, one EGF-like repeat, multiple sushi domains, and a transmembrane segment followed by a short cytoplasmic tail. Typically, the ligands for E-and P-Selectin are carbohydrates that contain a sialyl-Lewis X (sLeX) motif that appears on select O- and N-linked glycans and glycosphingolipids. Human E-Selectin is 95-115 kDa is size and expressed by vascular endothelium. It has a number of ligands, not all of which are shared between the human and rodent systems. For example human E-Selectin binds L-Selectin, CD44/HCELL and a poorly characterized pronase-insensitive ganglioside, none of which are recognized by mouse E-Selectin. By contrast, mouse E-Selectin binds E-Selectin Ligand whereas human E-Selectin does not. Other ligands identified for human E-Selectin include PSGL-1, CD43, Mac-1, CD66, Endoglycan and Podocalyxin-like protein. Human P-Selectin is 130-140 kDa in size, and expressed platelets and vascular endothelium. It primarily binds sLeX on O-glycans, including PSGL-1 and endoglycan. Functionally, Selectins induce leukocyte rolling on endothelial cell surfaces, an effect that activates neighboring alpha 2 beta 2/LFA-1 and slows leukocyte migration. Although the extracellular domains of human E- and P-Selectin show only 36% amino acid (aa) sequence identity, over the first 180 aa (out of >500 aa), E- and P- Selectin demonstrate 62% aa sequence identity.
  • Alternate Names:
    Eselectin; E-Selectin/P-Selectin; PSelectin
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Sample Type
  1. Neutrophil-mediated IL-6 receptor trans-signaling and the risk of chronic obstructive pulmonary disease and asthma
    Authors: N Farahi, E Paige, J Balla, E Prudence, RC Ferreira, M Southwood, SL Appleby, P Bakke, A Gulsvik, AA Litonjua, D Sparrow, EK Silverman, MH Cho, J Danesh, DS Paul, DF Freitag, ER Chilvers
    Hum. Mol. Genet., 2017;26(8):1584-1596.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
    Authors: Lauridsen H, Pober J, Gonzalez A
    FASEB J, 2014;28(3):1166-80.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC


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Isotype Controls
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Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 15  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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