Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of E‑Selectin/P‑Selectin (CD62E/P) in HUVEC Human Umbilical Vein Endothelial Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells stained with TNF-alpha was stained with Mouse Anti-Human E‑Selectin/P‑Selectin (CD62E/P) Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB6169F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: E-Selectin (CD62E)/P-Selectin (CD62P)
E-Selectin (CD62E) and P-Selectin (CD62P) are members of the Selectin/LECAM family of molecules. E- and P-Selectin are type I transmembrane glycoproteins that contain an N-terminal calcium-dependent C-type lectin domain, one EGF-like repeat, multiple sushi domains, and a transmembrane segment followed by a short cytoplasmic tail. Typically, the ligands for E-and P-Selectin are carbohydrates that contain a sialyl-Lewis X (sLeX) motif that appears on select O- and N-linked glycans and glycosphingolipids. Human E-Selectin is 95-115 kDa is size and expressed by vascular endothelium. It has a number of ligands, not all of which are shared between the human and rodent systems. For example human E-Selectin binds L-Selectin, CD44/HCELL and a poorly characterized pronase-insensitive ganglioside, none of which are recognized by mouse E-Selectin. By contrast, mouse E-Selectin binds E-Selectin Ligand whereas human E-Selectin does not. Other ligands identified for human E-Selectin include PSGL-1, CD43, Mac-1, CD66, Endoglycan and Podocalyxin-like protein. Human P-Selectin is 130-140 kDa in size, and expressed platelets and vascular endothelium. It primarily binds sLeX on O-glycans, including PSGL-1 and endoglycan. Functionally, Selectins induce leukocyte rolling on endothelial cell surfaces, an effect that activates neighboring alpha 2 beta 2/LFA-1 and slows leukocyte migration. Although the extracellular domains of human E- and P-Selectin show only 36% amino acid (aa) sequence identity, over the first 180 aa (out of >500 aa), E- and P- Selectin demonstrate 62% aa sequence identity.
Citations for Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Neutrophil-mediated IL-6 receptor trans-signaling and the risk of chronic obstructive pulmonary disease and asthma
Authors: N Farahi, E Paige, J Balla, E Prudence, RC Ferreira, M Southwood, SL Appleby, P Bakke, A Gulsvik, AA Litonjua, D Sparrow, EK Silverman, MH Cho, J Danesh, DS Paul, DF Freitag, ER Chilvers
Hum. Mol. Genet., 2017;26(8):1584-1596.
Sample Types: Whole Cells
Applications: Flow Cytometry
A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
Authors: Lauridsen H, Pober J, Gonzalez A
FASEB J, 2014;28(3):1166-80.
Sample Types: Whole Cells
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