Human ECE-1 Biotinylated Antibody

Catalog #: BAF1784
1 Citation Datasheet / COA / SDS

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ECE-1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ECE-1 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human ECE-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ECE-1
Gln90-Trp770
Accession # P42892

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ECE‑1 (Catalog # 1784-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ECE-1

Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.

Long Name

Endothelin-converting Enzyme-1

Entrez Gene IDs

1889 (Human)

Alternate Names

EC 3.4.24; EC 3.4.24.71; ECE; ECE1; ECE-1; endothelin converting enzyme 1; endothelin-converting enzyme 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human ECE-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Endothelin-converting enzyme-1 degrades internalized somatostatin-14.
Authors: Roosterman D, Kempkes C, Cottrell GS, Padilla BE, Bunnett NW, Turck CW, Steinhoff M
Endocrinology, 2008-02-14;149(5):2200-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC