Human ECE-1 Antibody

Catalog # Availability Size / Price Qty
AF1784
AF1784-SP
Detection of ECE‑1 in MCF‑7 Human Cell Line by Flow Cytometry.
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Product Details
Citations (7)
FAQs
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Human ECE-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ECE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human ECE-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ECE-1
Gln90-Trp770
Accession # P42892
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ECE‑1 (Catalog # 1784-ZN)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human ECE‑1 (Catalog # 1784‑ZN), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of ECE‑1 in MCF‑7 Human Cell Line by Flow Cytometry. View Larger

Detection of ECE‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Human ECE‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1784, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunohistochemistry ECE‑1 in Human Kidney. View Larger

ECE‑1 in Human Kidney. ECE‑1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ECE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1784) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ECE-1

Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.

References
  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341.
  3. Schweizer, A. et al. (1997) Biochem. J. 328:871.
  4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.
Long Name
Endothelin-converting Enzyme-1
Entrez Gene IDs
1889 (Human)
Alternate Names
EC 3.4.24; EC 3.4.24.71; ECE; ECE1; ECE-1; endothelin converting enzyme 1; endothelin-converting enzyme 1

Product Datasheets

Citations for Human ECE-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Retinal genes are differentially expressed in areas of primary versus secondary degeneration following partial optic nerve injury
    Authors: W Chiha, CJ LeVaillant, CA Bartlett, AW Hewitt, PE Melton, M Fitzgerald, AR Harvey
    PLoS ONE, 2018;13(2):e0192348.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Endothelin-converting enzymes degrade ?-synuclein and are reduced in dementia with Lewy bodies
    Authors: JS Miners, S Love
    J. Neurochem, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Endothelin-converting enzyme-1 (ECE-1) is post-transcriptionally regulated by alternative polyadenylation.
    Authors: Whyteside A, Turner A, Lambert D
    PLoS ONE, 2014;9(1):e83260.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Agonist-biased trafficking of somatostatin receptor 2A in enteric neurons.
    Authors: Zhao P, Canals M, Murphy J, Klingler D, Eriksson E, Pelayo J, Hardt M, Bunnett N, Poole D
    J Biol Chem, 2013;288(36):25689-700.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain.
    Authors: Wang S, Wang R, Chen L, Bennett DA, Dickson DW, Wang DS
    J. Neurochem., 2010;115(1):47-57.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Endosomal endothelin-converting enzyme-1: a regulator of beta-arrestin-dependent ERK signaling.
    Authors: Cottrell GS, Padilla BE, Amadesi S, Poole DP, Murphy JE, Hardt M, Roosterman D, Steinhoff M, Bunnett NW
    J. Biol. Chem., 2009;284(33):22411-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins.
    Authors: Padilla BE, Cottrell GS, Roosterman D, Pikios S, Muller L, Steinhoff M, Bunnett NW
    J. Cell Biol., 2007;179(5):981-97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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