Detection of Human EDNRB/Endothelin R Type B by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, A172 human glioblastoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EDNRB/Endothelin R Type B at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of EDNRB/Endothelin R Type B in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Human EDNRB/Endothelin R Type B Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4496, filled histogram) or isotype control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
EDNRB/Endothelin R Type B in A172 Human Cell Line.
EDNRB/Endothelin R Type B was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EDNRB/Endothelin R Type B
EDNRB (Endothelin B Receptor) is a member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2, and 3 and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64-Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa sequence identity with mouse and canine EDNRB, respectively.
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