Human EDNRB/Endothelin R Type B Antibody

Catalog # Availability Size / Price Qty
Detection of EDNRB/Endothelin R Type B in A172 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
Supplemental Products

Human EDNRB/Endothelin R Type B Antibody Summary

Species Reactivity
Detects human EDNRB in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human EDNRB
Accession # P24530
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of EDNRB/Endothelin R Type B antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of EDNRB/Endothelin R Type B in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Flow Cytometry Detection of EDNRB antibody in HEK293 Human Cell Line Transfected with Human EDNRB and eGFP antibody by Flow Cytometry. View Larger

Detection of EDNRB in HEK293 Human Cell Line Transfected with Human EDNRB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human EDNRB or (B) irrelevant protein and eGFP was stained with Sheep Anti-Human EDNRB Affinity Purified Polyclonal Antibody (Catalog # AF4496) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDNRB/Endothelin R Type B

EDNRB (Endothelin B Receptor) is a member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2, and 3 and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64-Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa sequence identity with mouse and canine EDNRB, respectively.

Long Name
Endothelin Receptor Type B
Entrez Gene IDs
1910 (Human); 13618 (Mouse); 50672 (Rat)
Alternate Names
ABCDS; EDNRB; endothelin B receptor; Endothelin receptor non-selective type; endothelin receptor type B; ETB; ET-B; ETBR; ET-BR; ETRB; HSCR; HSCR2; WS4A

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Citation for Human EDNRB/Endothelin R Type B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human pluripotent stem cells identify molecular targets of trisomy 12 in chronic lymphocytic leukemia patients
    Authors: JC Reid, D Golubeva, AL Boyd, CG Hollands, C Henly, L Orlando, A Leber, J Hébert, F Morabito, G Cutrona, L Agnelli, M Gentile, M Ferrarini, A Neri, B Leber, M Bhatia
    Cell Reports, 2021;34(11):108845.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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