Human EG-VEGF/PK1 Biotinylated Antibody

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BAF1209
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Human EG-VEGF/PK1 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human EG‑VEGF/PK1 in ELISAs and Western blots. In sandwich immunoassays, approximately 80% cross-reactivity with recombinant rat EG-VEGF is observed and 60% cross-reactivity with recombinant mouse EG-VEGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EG‑VEGF/PK1 (R&D Systems, Catalog # 1209-EV)
Ala20-Phe105
Accession # P58294
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human EG-VEGF/PK1 (Catalog # 1209-EV)

Human EG-VEGF/PK1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human EG‑VEGF/PK1 Antibody (Catalog # MAB12091)

Standard: Recombinant Human EG-VEGF/PK1 Protein (Catalog # 1209-EV)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EG-VEGF/PK1

Endocrine gland-derived vascular endothelial growth factor (EG-VEGF), also called prokineticin 1 (PK1), is a member of the prokineticin family of secreted proteins that share a common structural motif containing ten conserved cysteine residues that form five pairs of disulfide bonds (1, 2). Members of this family include the mammalian EG-VEGF/PK1 and PK2, as well as the venom protein A (VPRA) from the venom of black mamba snake and the frog Bombina variegata, Bv8 (1). Human EG-VEGF precursor is a 105 amino acid (aa) residue protein with a 19 aa signal peptide that is cleaved to yield a 86 aa mature protein (1, 2). EG-VEGF is expressed in multiple tissues including the gastrointestinal (GI) tract and steroidogenic glands (testis, ovary, placenta and adrenal glands). EG-VEGF has been shown to potently stimulate the contraction of GI smooth muscle. In addition, EG-VEGF is a tissue-specific angiogenic factor that exhibits biological activities similar to that of VEGF on select cells. It induces the proliferation, migration, and fenestration in cultured endocrine gland-derived capillary endothelial cells. EG-VEGF binds to and activates two closely related G protein-coupled receptors, EG-VEGF/PK1-R1 and EG-VEGF/PK2-R2 (3, 4). Activation of the receptors leads to stimulation of phosphoinositide turnover and activation of p44/p42 MAP kinase signaling pathways.

References
  1. Li, M. et al. (2001) Mol. Pharmacol. 59:692.
  2. LeCouter, J. et al. (2001) Nature 412:877.
  3. Lin, D. et al. (2002) J. Biol. Chem. 277:19276.
  4. Masuda, Y. et al. (2002) Biochem. Biophys. Res. Commun. 293:396.
Long Name
Endocrine Gland-derived Vascular Endothelial Growth Factor
Entrez Gene IDs
84432 (Human); 246691 (Mouse); 192205 (Rat)
Alternate Names
EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1

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