Human EGFR Quantikine ELISA Kit

R&D Systems | Catalog # DEGFR0

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Human Milk (50 µL)

Sensitivity

0.036 ng/mL

Assay Range

0.313-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Human Milk)
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Product Summary for Human EGFR Quantikine ELISA Kit

The Quantikine Human EGF R immunoassay is a 4.5 hour solid phase ELISA designed to measure EGF R in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human EGF R and has been shown to accurately quantitate the recombinant factor. Results obtained using natural EGF R showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human EGF R.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human EGF R

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 1.65 5.08 10.6 1.63 5.00 10.0
Standard Deviation 0.094 0.170 0.507 0.144 0.416 0.816
CV% 5.7 3.3 4.8 8.8 8.3 8.2

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 1.69 5.33 11.2 1.70 5.21 10.4
Standard Deviation 0.072 0.199 0.616 0.161 0.522 1.03
CV% 4.3 3.7 5.5 9.4 10.0 10.0

Recovery for Human EGFR Quantikine ELISA Kit

The recovery of EGF R spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 86-106
EDTA Plasma (n=4) 99 91-108
Heparin Plasma (n=4) 101 92-111
Serum (n=4) 101 89-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of EGF R were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human EGF R/ErbB1 ELISA Linearity

Scientific Data Images for Human EGFR Quantikine ELISA Kit

Human EGF R/ErbB1 ELISA Cell Culture Supernate/Human Milk Standard Curve

Human EGF R/ErbB1 ELISA Cell Culture Supernate/Human Milk Standard Curve

Human EGF R/ErbB1 ELISA Serum/Plasma Standard Curve

Human EGF R/ErbB1 ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EGFR

EGFR (epidermal growth factor receptor), also known as HER-1, ErbB1, or ErbB, is a transmembrane receptor tyrosine kinase that binds a subset of the EGF family ligands including EGF, Amphiregulin, TGF-alpha, Betacellulin, Epiregulin, HB-EGF, and Epigen. Ligand binding induces EGFR homodimerization as well as heterdimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs. Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

Additional EGFR Products

Product Documents for Human EGFR Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human EGFR Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human EGFR Quantikine ELISA Kit

Customer Reviews for Human EGFR Quantikine ELISA Kit (2)

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  • Human EGFR Quantikine ELISA Kit
    Name: Yuki Saito
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 12/18/2025
  • Human EGFR Quantikine ELISA Kit
    Name: Rahul Mallick
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Verified Customer | Posted 08/26/2020

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Protocols

View specific protocols for Human EGFR Quantikine ELISA Kit (DEGFR0):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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