Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
FAB9577B-100
Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.
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Product Details
Citations (1)
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Reviews (1)

Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGFR based on Cetuximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Cetuximab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu1
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human EGF R/ErbB1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of EGF R/ErbB1 antibody in A431 human epithelial carcinoma cell line antibody by Flow Cytometry. View Larger

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B, filled histogram) or irrelevant biotinylated antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry EGFR antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

EGFR in A431 Human Cell Line. EGFR was detected in unfixed live A431 human epithelial carcinoma cell line using Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EGFR

Cetuximab is a biosimilar directed against the extracellular domain of EGFR. The biotinylated antibody can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.

Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Product Datasheets

Citation for Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window
    Authors: T Egnuni, N Ingram, I Mirza, PL Coletta, JR McLaughlan
    Pharmaceutics, 2021;13(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Reviews for Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody

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Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
By Anonymous on 09/29/2020
Application: Flow Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Red histogram: HEK293 cells
Blue histogram: HEK293 cells transduced to express a protein containing the EGFR tag