Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
View Larger
Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B, filled histogram) or irrelevant biotinylated antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.
View Larger
EGFR in A431 Human Cell Line. EGFR was detected in unfixed live A431 human epithelial carcinoma cell line using Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EGFR
Cetuximab is a biosimilar directed against the extracellular domain of EGFR. The biotinylated antibody can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.
Product Datasheets
Citation for Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window
Authors: T Egnuni, N Ingram, I Mirza, PL Coletta, JR McLaughlan
Pharmaceutics, 2021;13(10):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsStaining Reagents
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
Average Rating: 4 (Based on 1 Review)
Have you used Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Red histogram: HEK293 cells
Blue histogram: HEK293 cells transduced to express a protein containing the EGFR tag
