Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody

Name: Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
Brand: R&D Systems
SKU: FAB9577B-100
Rating: 4 (1 reviews)

Catalog #: FAB9577B
3 Citations 1 Review Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

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FAB9577B-100

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Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human EGFR based on Cetuximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Cetuximab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu1

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human EGF R/ErbB1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B, filled histogram) or irrelevant biotinylated antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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EGFR in A431 Human Cell Line. EGFR was detected in unfixed live A431 human epithelial carcinoma cell line using Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: EGFR

Cetuximab is a biosimilar directed against the extracellular domain of EGFR. The biotinylated antibody can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Regulatory programs of B-cell activation and germinal center reaction allow B-ALL escape from CD19 CAR T-cell therapy
Authors: NG Im, A Guillaumet, M Wal, AJ Rogers, J Frede, CC Havig, J Yang, P Anand, SK Stegmann, JM Waldschmid, N Sotudeh, L Niu, J Voisine, MR Schweiger, C Grassberge, JG Lohr, B Knoechel
Oncogene, 2022;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window
Authors: T Egnuni, N Ingram, I Mirza, PL Coletta, JR McLaughlan
Pharmaceutics, 2021;13(10):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome
Authors: V Vavassori, E Mercuri, GE Marcovecch, MC Castiello, G Schiroli, L Albano, C Margulies, F Buquicchio, E Fontana, S Beretta, I Merelli, A Cappelleri, PM Rancoita, V Lougaris, A Plebani, M Kanariou, A Lankester, F Ferrua, E Scanziani, C Cotta-Ramu, A Villa, L Naldini, P Genovese
Embo Molecular Medicine, 2021;0(0):e13545.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay