Human EMR2 Antibody

Catalog # Availability Size / Price Qty
Cell Adhesion Mediated by EMR2 and Neutralization by Human EMR2 Antibody.
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Product Details
Citations (2)
Supplemental Products

Human EMR2 Antibody Summary

Species Reactivity
Detects human EMR2 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 1% cross-reactivity with recombinant mouse EMR1.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human EMR2
Gln24-Gln478 (Gln24 predicted)
Accession # NP_038475
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Measured by its ability to neutralize EMR2-mediated adhesion of the NIH‑3T3 mouse embryonic fibroblast cell line. Stacey, M. et al. (2003) Blood 102:29161. The Neutralization Dose (ND50) is typically 1.5-6 µg/mL in the presence of 1.5 µg/mL Recombinant Human EMR2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Adhesion Mediated by EMR2 and Neutralization by Human EMR2 Antibody. View Larger

Cell Adhesion Mediated by EMR2 and Neutralization by Human EMR2 Antibody. Recombinant Human EMR2 (Catalog # 4894-EM), immobilized onto a microplate, supports the adhesion of the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human EMR2 (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894). The ND50 is typically 1.5-6 µg/mL.

Western Blot Detection of Human EMR2 antibody by Western Blot. View Larger

Detection of Human EMR2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4894) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMR2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMR2

EMR2 (EGF-like module-containing mucin-like receptor 2; designated CD312) is a glycoprotein belonging to the EGF-TM7 family of adhesion-type class B 7‑transmembrane (TM) receptors. EGF-like sequences within long extracellular N-termini, and a GPS (G-protein proteolytic site) domain are characteristic of this family, which is mainly expressed on cells of the immune system (1, 2). The human EMR2 cDNA encodes an 823 amino acid (aa) protein with five EGF-like domains within the first 250 aa, followed by a mucin-like stalk, a GPS domain (aa 479-530) and a 7-TM sequence (aa 531-785). The GPS domain is the site of autocatalytic cleavage, forming two cleaved portions that remain non-covalently attached as a heterodimer (1, 3). Of the first 290 aa of human EMR2, 284 aa (97%) are identical with family member CD97, likely due to gene duplication (2). The portion of human EMR2 N-terminal to the GPS domain (aa 1-478) shares 64%, 59%, 48% and 45% aa identity with corresponding regions of canine EMR2, equine EMR2, mouse CD97 and rat CD97, respectively. Alternate splicing of EMR2 creates isoforms that contain 2-5 EGF-like domains. Only the 5-EGF form contains EGF4, which is necessary for calcium-dependent binding of the EMR2/CD97 ligand, chondroitin sulfate (CS) (2, 4‑6). None of the isoforms engage the CD97 ligand, CD55 (DAF). EMR2 is restricted to myeloid cells (1, 2). EMR2 expression increases as monocytes differentiate into macrophages, and decreases with differentiation into dendritic cells (5). Activation increases neutrophil EMR2 expression (5). EMR2 localizes to the leading edge of migrating neutrophils and plays an important role in migration, adhesion and superoxide production (7). It is also thought to facilitate specific interaction of myeloid cells with peripheral B lymphocytes which express CS (6).

  1. Kwakkenbos, M.J. et al. (2004) Immunogenetics 55:655.
  2. Lin, H.-H. et al. (2000) Genomics 67:188.
  3. Lin, H.-H. et al. (2004) J. Biol. Chem. 279:31823.
  4. Stacey, M. et al. (2003) Blood 102:2916.
  5. Chang G.-W. et al. (2007) Biochem. Biophys. Res. Commun. 353:133.
  6. Kwakkenbos, M.J. et al. (2005) J. Leukoc. Biol. 77:112.
  7. Yona, S. et al. (2008) FASEB J. 22:741.
Long Name
EGF-like Module Containing, Mucin-like, Hormone Receptor-like 2
Entrez Gene IDs
30817 (Human)
Alternate Names
CD312; DKFZp781B135; egf-like module containing, mucin-like, hormone receptor-like 2; egf-like module containing, mucin-like, hormone receptor-like sequence 2; EGF-like module receptor 2; EGF-like module-containing mucin-like hormone receptor-like 2; EMR2

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Citations for Human EMR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Factor H-related protein 1 (FHR-1) is associated with atherosclerotic cardiovascular disease
    Authors: S Irmscher, SLH Zipfel, LD Halder, L Ivanov, A Gonzalez-D, C Waldeyer, M Seiffert, FJ Brunner, M von der He, I Löschmann, S Wulf, D Czamara, N Papac-Mili, O Strau beta, S Lorkowski, H Reichenspu, MV Holers, NK Banda, T Zeller, EB Binder, CJ Binder, T Wiech, PF Zipfel, C Skerka
    Scientific Reports, 2021;11(1):22511.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. G Protein-Coupling of Adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and Activation of G Protein Signalling by an Anti-EMR2 Antibody
    Authors: N Bhudia, S Desai, N King, N Ancellin, D Grillot, AA Barnes, SJ Dowell
    Sci Rep, 2020;10(1):1004.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation


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