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Human Engrailed-2 Antibody

Catalog #: MAB2600 Datasheet / COA / SDS

Discontinued Product

MAB2600 has been discontinued.
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Human Engrailed-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Engrailed-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Engrailed-1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 279122
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Engrailed-2
Ser118-Glu333
Accession # P19622
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Engrailed‑2
Immunocytochemistry
8-25 µg/mL
Immersion fixed human dopaminergic neuronal precursors derived from embryonic stem cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Engrailed-2

Engrailed-2 is a homeodomain-containing protein that has been implicated in the control of pattern formation during development of the central nervous system.

Entrez Gene IDs
2020 (Human)
Alternate Names
AUTS10; EN2; engrailed homeobox 2; engrailed homolog 2; Engrailed2; Engrailed-2; Homeobox protein en-2; homeobox protein engrailed-2; hu-En-2

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