Human ENPP-1 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human ENPP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human ENPP-2 and recombinant mouse ENPP-2 is observed after removal of cross-reactivity with human ENPP-2.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ENPP-2-1-2
    Asp49-Trp144 (ENPP-2), Val191-Leu591 (ENPP-1), Asn532-Ile863 (ENPP-2). Cross-reactivity with human ENPP-2 was removed from the final product.
    Accession # Q13822 (ENPP-2) P22413 (ENPP-1)
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ENPP‑1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human ENPP‑1 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6136G, filled histogram) or isotype control antibody (Catalog # IC016G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ENPP-1
Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP-1) is a transmembrane glycoprotein that hydrolyzes nucleotides and nucleotide derivatives with the formation of nucleotide-5’-monophosphates. It is inserted into the plasma membrane by an N‑terminal transmembrane domain. Human ENPP-1 has a small N-terminal cytoplasmic domain and a large C-terminal region containing two somatomedin B-like domains, a catalytic domain and a nuclease-like domain in the extracellular space (1). Defects in the ENPP-1 gene cause arterial calcification and bone mineralization abnormalities (2). ENPP-1 polymorphism or overexpression is also associated with obesity, type II diabetes and insulin resistance, which makes modulation of ENPP-1 activity one of the targets to treat insulin resistance and related diseases (1).
  • References:
    1. Goldfine, I.D. et al. (2010) Endocrine Reviews. 29:62.
    2. Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. 99:9445.
  • Long Name:
    Ectonucleotide Pyrophosphatase/Phosphodiesterase 1
  • Entrez Gene IDs:
    5167 (Human)
  • Alternate Names:
    ectonucleotide pyrophosphatase/phosphodiesterase 1; E-NPP 1; ENPP1; Ly-41 Antigen; M6S1; M6S1NPP1; Membrane component chromosome 6 surface marker 1; membrane component, chromosome 6, surface marker 1; NPP1; NPPS; NPPSalkaline phosphodiesterase 1; PC1; PC-1; PCA1; PCA1ARHR2; PDNP1; PDNP1ectonucleotide pyrophosphatase/phosphodiesterase family member 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; plasma-cell membrane glycoprotein 1; Plasma-cell membrane glycoprotein PC-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC016G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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