Human Enteropeptidase/Enterokinase Antibody

Catalog # Availability Size / Price Qty
MAB1585
MAB1585-SP
Product Details
Citations (6)
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Human Enteropeptidase/Enterokinase Antibody Summary

Species Reactivity
Human
Specificity
Detects human Enteropeptidase/Enterokinase in direct ELISAs and Western blots. It recognizes all EK forms containing the heavy chain including the single chain precursor and the mature heterodimer consisting of a heavy and light chain. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) Factor X and rhThrombin is observed.
Source
Monoclonal Mouse IgG2A Clone # 190034
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Enteropeptidase/Enterokinase
Leu41-His1019
Accession # NP_002763
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Enteropeptidase/Enterokinase (Catalog # 1585-SE) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Enteropeptidase/Enterokinase (Catalog # 1585-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Enteropeptidase/Enterokinase

Enterokinase (EK), also called enteropeptidase and TMPRSS15 (gene name), initiates activation of pancreatic proteases by converting trypsinogen to trypsin, which in turn activates chymotrypsin, carboxypeptidases and elastases. Located in intestinal brush border, it is a disulfide bond-linked dimer of the heavy and light chains, which are derived from the same single-chain precursor. The heavy chain consists of a short cytoplamic tail, a transmembrane, a SEA, a SRCR, a MAM, two CUB and two LDL-receptor class A domains. The light chain contains the catalytic domain of trypsin-like serine proteases.

Entrez Gene IDs
5651 (Human)
Alternate Names
EC 3.4.21; EC 3.4.21.9; Enterokinase; Enteropeptidase; ENTK; ENTKenterokinase; MGC133046; protease, serine, 7 (enterokinase); PRSS7; PRSS7enteropeptidase; Serine protease 7; TMPRSS15; Transmembrane protease serine 15; transmembrane protease, serine 15

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Citations for Human Enteropeptidase/Enterokinase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Authors: Zelinka C, Scott M, Volkov L, Fischer A
    PLoS ONE, 2012-09-04;7(9):e44477.
  2. Intra-amniotic transplantation of brain-derived neurotrophic factor-modified mesenchymal stem cells treatment for rat fetuses with spina bifida aperta
    Authors: Ma W, Wei X, Gu H et al.
    Stem cell research & therapy
  3. Fragile X Mental Retardation Protein expression in the retina is regulated by light.
    Authors: Guimaraes-Souza EM, Perche O, Morgans CW et al.
    Exp Eye Res
  4. Brn3a controls the soma localization and axonal extension patterns of developing spinal dorsal horn neurons
    Authors: Nishida, K;Matsumura, S;Uchida, H;Abe, M;Sakimura, K;Badea, TC;Kobayashi, T;
    PloS one
  5. IGFBPL1 is a master driver of microglia homeostasis and resolution of neuroinflammation in glaucoma and brain tauopathy
    Authors: Pan, L;Cho, KS;Wei, X;Xu, F;Lennikov, A;Hu, G;Tang, J;Guo, S;Chen, J;Kriukov, E;Kyle, R;Elzaridi, F;Jiang, S;Dromel, PA;Young, M;Baranov, P;Do, CW;Williams, RW;Chen, J;Lu, L;Chen, DF;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Intracellular co-localization of trypsin-2 and matrix metalloprotease-9: possible proteolytic cascade of trypsin-2, MMP-9 and enterokinase in carcinoma.
    Authors: Vilen ST, Nyberg P, Hukkanen M, Sutinen M, Ylipalosaari M, Bjartell A, Paju A, Haaparanta V, Stenman UH, Sorsa T, Salo T
    Exp. Cell Res., 2007-11-12;314(4):914-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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