Human ErbB2/Her2 PE-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human ErbB2/Her2 in ELISAs and Western blots. In ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4.
  • Source
    Monoclonal Mouse IgG2B Clone # 191924
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2
    Accession # P04626
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 PE‑conjugated Monoclonal Antibody (Catalog # FAB1129P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).

  • References:
    1. Coussens, L. et. al. (1985) Science 230:1132.
    2. Yamamoto, T. et. al. (1986) Nature 319:230.
    3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
    4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
    5. Carraway, K.L. 3rd et. al.  (1994) J. Biol. Chem. 269:14303.
    6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
    7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
    8. Schlessinger, J. (2000) Cell 103:211.
    9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
    10. Daly, R.J. (1999) Growth Factors 16:255.
  • Long Name:
    Receptor Tyrosine Protein Kinase ErbB2
  • Entrez Gene IDs:
    2064 (Human); 13866 (Mouse); 24337 (Rat)
  • Alternate Names:
    CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; HER2; HER-2; HER2EC; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 3 of 3
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Sample Type
  1. Growth-factor-driven rescue to receptor tyrosine kinase (RTK) inhibitors through Akt and Erk phosphorylation in pediatric low grade astrocytoma and ependymoma.
    Authors: Sie M, den Dunnen W, Lourens H, Meeuwsen-de Boer T, Scherpen F, Zomerman W, Kampen K, Hoving E, de Bont E
    PLoS ONE, 2015;10(3):e0122555.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Side-population cells in luminal-type breast cancer have tumour-initiating cell properties, and are regulated by HER2 expression and signalling.
    Authors: Nakanishi T, Chumsri S, Khakpour N, Brodie AH, Leyland-Jones B, Hamburger AW, Ross DD, Burger AM
    Br. J. Cancer, 2010;102(5):815-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Lapatinib (Tykerb, GW572016) reverses multidrug resistance in cancer cells by inhibiting the activity of ATP-binding cassette subfamily B member 1 and G member 2.
    Authors: Dai CL, Tiwari AK, Wu CP, Su XD, Wang SR, Liu DG, Ashby CR, Huang Y, Robey RW, Liang YJ, Chen LM, Shi CJ, Ambudkar SV, Chen ZS, Fu LW
    Cancer Res., 2008;68(19):7905-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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