Human ErbB2/Her2 Antibody MAB1129-100: R&D Systems

Human ErbB2/Her2 Antibody

(4 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human ErbB2/Her2 in ELISAs and Western blots. In ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4.
  • Source
    Monoclonal Mouse IgG2B Clone # 191924
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2
    Thr23-Thr652
    Accession # P04626
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER)
    under non-reducing conditions only
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
    • Human ErbB2/Her2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human ErbB2/Her2 Antibody (Catalog # MAB1129)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human ErbB2/Her2 Biotinylated Antibody (Catalog # BAF1129)
  • ELISA Standard
     
    Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF (Catalog # 1129-ER)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
ErbB2/Her2 in MDA‑MB‑231 Human Cell Line. ErbB2/Her2 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
ErbB2/Her2 in Human Stomach. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane/cytoplasm in glans and villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ErbB2/Her2
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).
  • References:
    1. Coussens, L. et. al. (1985) Science 230:1132.
    2. Yamamoto, T. et. al. (1986) Nature 319:230.
    3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
    4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
    5. Carraway, K.L. 3rd et. al.  (1994) J. Biol. Chem. 269:14303.
    6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
    7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
    8. Schlessinger, J. (2000) Cell 103:211.
    9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
    10. Daly, R.J. (1999) Growth Factors 16:255.
  • Long Name:
    Receptor Tyrosine Protein Kinase ErbB2
  • Entrez Gene IDs:
    2064 (Human); 13866 (Mouse); 24337 (Rat)
  • Alternate Names:
    CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Detection of Circulating Tumour Cells in Urothelial Cancers and Clinical Correlations: Comparison of Two Methods
    Authors: E Fina, A Necchi, S Bottelli, C Reduzzi, S Pizzamigli, C Iacona, MG Daidone, P Verderio, V Cappellett
    Dis. Markers, 2017;2017(0):3414910.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome
    Authors: TS Klann, JB Black, M Chellappan, A Safi, L Song, IB Hilton, GE Crawford, TE Reddy, CA Gersbach
    Nat. Biotechnol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. HER2 and EGFR overexpression support metastatic progression of prostate cancer to bone
    Cancer Res., 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Ctyometry
  4. Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
    Authors: Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA
    Assay Drug Dev Technol, 2010;8(1):27-36.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
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Isotype Controls
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Average Rating: 5 (Based on 2 reviews)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Human Anonymous 05/19/2017
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Summary

ApplicationFlow Cytometry
Sample TestedA431 human epithelial carcinoma cell line
SpeciesHuman
  Excellent
  Anonymous 04/24/2017
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