Intracellular Staining by Flow Cytometry
|Detection of FATP1 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human FATP1 PE‑conjugated Monoclonal Antibody (Catalog # IC3304P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Intracellular Molecules.|
FATP1 (Fatty Acid Transport Protein 1), also known as SLC27A1, is a 63-64 kDa protein that belongs to the ATP-dependent AMP-binding enzyme family. A type I transmembrane protein, FATP1 is one of six mammalian FATPs that catalyze an ATP-dependent esterification of long-chain fatty acids. FATP1 is expressed in cells with high rates of fatty acid uptake for storage of energy, principally in brown and white adipocytes, plus skeletal muscle myocytes. FATP1 likely functions as a homodimer, and is found in multiple locations such as cytoplasm, in intracellular membranes, and in the plasma membrane in response to insulin (in adipocytes). Full length FATP1 (amino acids 1-646) shares 89% amino acid sequence identity with mouse FATP1.
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