Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Fish - Salmo salar (Atlantic Salmon)

Applications

Validated:

Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 308420
View all FATP1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human FATP1
Met1-Leu646
Accession # Q6PCB7

Specificity

Detects human FATP1 in cell transfectants but not non-transfected parent cells in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FATP1 Antibody

FATP1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

FATP1 in HeLa Human Cell Line.

FATP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human FATP1 Monoclonal Antibody (Catalog # MAB3304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human FATP1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FATP1

FATP1 is a plasma membrane protein with 6 transmembrane domains. It is involved in translocation of long-chain fatty acids across the plasma membrane. FATP1 is expressed in muscle, intestine, and adipose tissue.

Long Name

Fatty Acid Transport Protein 1

Alternate Names

SLC27A1

Entrez Gene IDs

376497 (Human); 26457 (Mouse); 94172 (Rat)

Gene Symbol

SLC27A1

UniProt

Q6PCB7

Additional FATP1 Products

Product Documents for Human FATP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FATP1 Antibody

For research use only

Related Research Areas

Adipogenesis Markers
Obesity

Citations for Human FATP1 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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