Human Fc gamma  RIIIA/B (CD16) Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1597
Product Details
Citations (1)
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Human Fc gamma  RIIIA/B (CD16) Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fc gamma RIIIA/B (CD16) in Western blots. In this format, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIB (R&D Systems, Catalog # 1597-FC)
Thr20-Gln208
Accession # O75015
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Fc gamma  RIIIB/CD16b (Catalog # 1597-FC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIII (CD16)

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta  chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.

References
  1. van de Winkel, J, and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Gauchat, G.J. et al. (1996) J. Immunol. 157:1184.
Long Name
Fc gamma Receptor III
Entrez Gene IDs
14131 (Mouse); 102140945 (Cynomolgus Monkey)
Alternate Names
CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

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Citation for Human Fc gamma  RIIIA/B (CD16) Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Characterization of Endogenous Human Fc gamma RIII by Mass Spectrometry Reveals Site, Allele and Sequence Specific Glycosylation*
    Authors: Nathaniel Washburn, Robin Meccariello, Jay Duffner, Kristen Getchell, Kimberly Holte, Thomas Prod'homme et al.
    Molecular & Cellular Proteomics

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