Human Fetuin A/AHSG Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1184
Product Details
Citations (1)
FAQs
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Human Fetuin A/AHSG Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fetuin A in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity is observed with rmFetuin A.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fetuin A
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Fetuin A/AHSG (Catalog # 1184-PI)

Human Fetuin A/AHSG Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Fetuin A/AHSG Antibody (Catalog # MAB11841)

Standard: Recombinant Human Fetuin A/AHSG Protein, CF (Catalog # 1184-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin A/AHSG

Human Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (1, 2). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (3). The major form of plasma Fetuin A corresponds to a disulfide bond-linked two chains derived from the single chain (4). Human Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300 - 600 μg/mL), which fall significantly (30 - 50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (6, 7). The purified rhFetuin A corresponds to the single chain, which can be converted to the two-chain form by rhFurin (R&D Systems, Catalog # 1503-SE) in vitro. However, the conversion does not enhance its inhibitory activity against rhCathpesin V, a cysteine protease.

References
  1. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
  2. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
  3. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
  4. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta. 671:78.
  5. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
  6. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
  7. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
Long Name
alpha-2-HS Glycoprotein
Entrez Gene IDs
197 (Human); 11625 (Mouse); 25373 (Rat)
Alternate Names
A2HS; AHS; AHSG; alpha-2-HS-glycoprotein; alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; FETUAba-alpha-2-glycoprotein; Fetuin A; fetuin-A; HSGA

Product Datasheets

Citation for Human Fetuin A/AHSG Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008;4(2):e41.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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