Detection of Human Fetuin A/AHSG by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fetuin A/AHSG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Fetuin A/AHSG in Human Liver.
Fetuin A/AHSG was detected in immersion fixed paraffin-embedded sections of human liver array using Goat Anti-Human Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1184) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fetuin A/AHSG
Human Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (1, 2). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (3). The major form of plasma Fetuin A corresponds to a disulfide bond-linked two chains derived from the single chain (4). Human Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (6, 7). The purified rhFetuin A corresponds to the single chain, which can be converted to the two-chain form by rhFurin (R&D Systems, Catalog # 1503-SE) in vitro. However, the conversion does not enhance its inhibitory activity against rhCathpesin V, a cysteine protease.
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Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol.106:319.
Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta. 671:78.
Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
Mathews, S.T. et al. (2000) Mol. Cell Endocrinol.164:87.
Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
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