Human Fetuin A/AHSG Antibody

Catalog # Availability Size / Price Qty
Detection of Human Fetuin A/AHSG by Western Blot.
2 Images
Product Details
Citations (3)
Supplemental Products

Human Fetuin A/AHSG Antibody Summary

Species Reactivity
Detects human Fetuin A in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Fetuin A and recombinant rat Fetuin A is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Fetuin A/AHSG
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Fetuin A/AHSG antibody by Western Blot. View Larger

Detection of Human Fetuin A/AHSG by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fetuin A/AHSG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Fetuin A/AHSG antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

Fetuin A/AHSG in Human Liver. Fetuin A/AHSG was detected in immersion fixed paraffin-embedded sections of human liver array using Goat Anti-Human Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1184) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin A/AHSG

Human Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (1, 2). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (3). The major form of plasma Fetuin A corresponds to a disulfide bond-linked two chains derived from the single chain (4). Human Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (6, 7). The purified rhFetuin A corresponds to the single chain, which can be converted to the two-chain form by rhFurin (R&D Systems, Catalog # 1503-SE) in vitro. However, the conversion does not enhance its inhibitory activity against rhCathpesin V, a cysteine protease.

  1. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
  2. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
  3. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
  4. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta. 671:78.
  5. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
  6. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
  7. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
Long Name
alpha-2-HS Glycoprotein
Entrez Gene IDs
197 (Human); 11625 (Mouse); 25373 (Rat)
Alternate Names
A2HS; AHS; AHSG; alpha-2-HS-glycoprotein; alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; FETUAba-alpha-2-glycoprotein; Fetuin A; fetuin-A; HSGA

Product Datasheets

Citations for Human Fetuin A/AHSG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Association of fetuin-A with incident type 2 diabetes: Results from the MONICA/KORA Augsburg study and a systematic meta-analysis
    Authors: C Sujana, C Huth, A Zierer, S Meesters, J Sudduth-Kl, W Koenig, C Herder, A Peters, B Thorand
    Eur. J. Endocrinol., 2018;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: Single Molecule Capture - Dete
  2. Mutations in the ABCC6 gene as a cause of generalized arterial calcification of infancy: genotypic overlap with pseudoxanthoma elasticum.
    Authors: Li, Qiaoli, Brodsky, Jill L, Conlin, Laura K, Pawel, Bruce, Glatz, Andrew C, Gafni, Rachel I, Schurgers, Leon, Uitto, Jouni, Hakonarson, Hakon, Deardorff, Matthew, Levine, Michael
    J Invest Dermatol, 2014;134(3):658-65.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Mutations in the GGCX and ABCC6 genes in a family with pseudoxanthoma elasticum-like phenotypes.
    Authors: Li Q, Grange DK, Armstrong NL, Whelan AJ, Hurley MY, Rishavy MA, Hallgren KW, Berkner KL, Schurgers LJ, Jiang Q, Uitto J
    J. Invest. Dermatol., 2009;129(3):553-63.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Fetuin A/AHSG Antibody

There are currently no reviews for this product. Be the first to review Human Fetuin A/AHSG Antibody and earn rewards!

Have you used Human Fetuin A/AHSG Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review