Human FGF-23 Antibody
Human FGF-23 Antibody Summary
Accession # Q9GZV9
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibroblast growth factor 23 (FGF-23) is a 30-32 kDa member of the FGF family, within a subfamily that also includes FGF-19 and FGF-21. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). FGF-19 subfamily members are highly diffusible molecules owing to their poor ECM/heparin sulfate binding and plasma-stabilizing intramolecular folds (2-4). Mature human FGF-23 contains an atypical (very low affinity) heparin binding site (aa 134-162), a proteolytic cleavage site (Arg179-Ser180), and multiple O-linked glycosylation sites with Thr178 being of particular importance (4-7). O-linked glycosylation at Thr178 blocks the cleavage of FGF-23, thereby preventing loss of FGF-23 activity (7, 8). Mature human FGF-23 shows 72% aa identity to mouse FGF-23 and is active on mouse cells (6). FGF-23 exerts its effects through a ternary complex that includes Klotho and an FGF receptor (FGF R4 or the "c" isoforms of FGF R1 or FGF R3). Klotho has a restricted distribution that limits FGF-23 activity (9-11). FGF-23 is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone, and circulatory volume loading. It functions as an endocrine phosphatonin by suppressing circulating phosphate levels (12). FGF-23 interaction with renal proximal tubular epithelium decreases the renal resorption of phosphate by downregulating phosphate transporters and by suppressing vitamin D production. It also decreases the intestinal absorption of phosphate (13).
- Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
- Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
- Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
- Harmer, N.J. et al. (2004) Biochemistry 43:629.
- Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
- Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
- Frishberg, Y. et al. (2007) J. Bone Miner. Res. 22:235.
- Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
- Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
- Urakawa, I. et al. (2006) Nature 444:770.
- Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
- Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
- Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human FGF-23 Antibody
There are currently no reviews for this product. Be the first to review Human FGF-23 Antibody and earn rewards!
Have you used Human FGF-23 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image